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Product Info Summary
| SKU: | A02642-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Dermatopontin/DPT Antibody Picoband®
View all Dermatopontin Antibodies
SKU/Catalog Number
A02642-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Dermatopontin/DPT Antibody Picoband® catalog # A02642-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dermatopontin/DPT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02642-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Dermatopontin recombinant protein (Position: Q19-V201).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02642-1 is reactive to DPT in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
20 kDa
Calculated molecular weight
33310 MW
Background of Dermatopontin
Dermatopontin is a protein that in humans is encoded by the DPT gene. Dermatopontin is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02642-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human placenta tissue, rat lung tissue, mouse lung tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Dermatopontin using anti-Dermatopontin antibody (A02642-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dermatopontin antigen affinity purified polyclonal antibody (Catalog # A02642-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dermatopontin at approximately 20KD. The expected band size for Dermatopontin is at 24KD.
Protein Target Info & Infographic
Gene/Protein Information For DPT (Source: Uniprot.org, NCBI)
Gene Name
DPT
Full Name
Dermatopontin
Weight
33310 MW
Superfamily
dermatopontin family
Alternative Names
Dermatopontin; Tyrosine-rich acidic matrix protein; TRAMP; DPT DPT TRAMP dermatopontin dermatopontin|tyrosine-rich acidic matrix protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DPT, check out the DPT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DPT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dermatopontin/DPT Antibody Picoband® (A02642-1)
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Customer Q&As
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3 Customer Q&As for Anti-Dermatopontin/DPT Antibody Picoband®
Question
I am looking for to test anti-Dermatopontin/DPT antibody A02642-1 on human testis for research purposes, then I may be interested in using anti-Dermatopontin/DPT antibody A02642-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The products we sell, including anti-Dermatopontin/DPT antibody A02642-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-25
Question
We are currently using anti-Dermatopontin/DPT antibody A02642-1 for mouse tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
The anti-Dermatopontin/DPT antibody (A02642-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-17
Question
Can you help my question with product A02642-1, anti-Dermatopontin/DPT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02642-1 anti-Dermatopontin/DPT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-12
