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Product Info Summary
| SKU: | A30477 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-SFRS4 Antibody
SKU/Catalog Number
A30477
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SFRS4 Antibody (Catalog# A30477). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SFRS4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30477)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human SFRS4. Immunogen sequence location: 111-160
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30477 is reactive to SRSF4 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
56678 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30477 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from LOVO cells, using SFRS4 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For SRSF4 (Source: Uniprot.org, NCBI)
Gene Name
SRSF4
Full Name
Serine/arginine-rich splicing factor 4
Weight
56678 MW
Superfamily
splicing factor SR family
Alternative Names
SRSF4;SFRS4;SRP75;Serine/arginine-rich splicing factor 4;Pre-mRNA-splicing factor SRP75;SRP001LB;Splicing factor; arginine/serine-rich 4 SRSF4 SFRS4, SRP75 serine and arginine rich splicing factor 4 serine/arginine-rich splicing factor 4|SR splicing factor 4|SRP001LB|pre-mRNA-splicing factor SRP75|splicing factor, arginine/serine-rich 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SRSF4, check out the SRSF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SRSF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SFRS4 Antibody (A30477)
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No publications found for A30477
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-SFRS4 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for left lobe of thyroid gland using anti-SFRS4 antibody A30477. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-03
Question
We are currently using anti-SFRS4 antibody A30477 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?
S. Zhang
Verified customer
Asked: 2017-12-11
Answer
The anti-SFRS4 antibody (A30477) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-11
Question
Does A30477 anti-SFRS4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Williams
Verified customer
Asked: 2015-05-28
Answer
As indicated on the product datasheet, A30477 anti-SFRS4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-05-28
