Product Info Summary
SKU: | EKC1780 |
---|---|
Size: | 1 kit, containing one 96-well plate and all necessary reagents |
Reactive Species: | Human, Mouse |
Application: | ELISA |
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Product info
Product Name
SFRS4 Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC1780
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The SFRS4 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SFRS4 protein expression profile in cells. The kit can be used for measuring the relative amounts of SFRS4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SFRS4.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
SFRS4 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1780)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1780 is reactive to SRSF4 in Human, Mouse samples
Application Guarantee
EKC1780 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Reagent | Quantity | Container |
---|---|---|
96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
10x TBS | 24 ml (10x) | Clear |
Quenching Buffer | 24 ml (1x) | Clear |
Blocking Buffer | 50 ml (1x) | Clear |
15x Wash Buffer | 50 ml (15x) | Clear |
100x Anti-SFRS4 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
Primary Antibody Diluent | 12 ml (1x) | Clear |
Ready-to-Use Substrate | 12 ml (1x) | Brown |
Stop Solution | 12 ml (1x) | Clear |
Crystal Violet Solution | 6ml (1x) | Glass |
SDS Solution | 24 ml (1x) | Clear |
Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
![boster box boster box](https://www.bosterbio.com/media/catalog/product/b/o/boster-box.png)
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Boster Kit Box
![ekc1780 sfrs4 colorimetric cell based elisa wb testing 1 ekc1780 sfrs4 colorimetric cell based elisa wb testing 1](https://www.bosterbio.com/media/catalog/product/e/k/ekc1780-sfrs4-colorimetric-cell-based-elisa-wb-testing-1.jpg)
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Western blot analysis of extracts from LOVO cells
Protein Target Info & Infographic
Gene/Protein Information For SRSF4 (Source: Uniprot.Org, NCBI)
Gene Name
SRSF4
Full Name
Serine/arginine-rich splicing factor 4
Weight
56678 MW
Superfamily
splicing factor SR family
Alternative Names
Pre-mRNA-splicing factor SRP75; serine/arginine-rich splicing factor 4; SFRS4; Splicing factor, arginine/serine-rich 4SRP001LB; SRP75SR splicing factor 4 SRSF4 SFRS4, SRP75 serine and arginine rich splicing factor 4 serine/arginine-rich splicing factor 4|SR splicing factor 4|SRP001LB|pre-mRNA-splicing factor SRP75|splicing factor, arginine/serine-rich 4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SRSF4, check out the SRSF4 Infographic
![SRSF4 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SRSF4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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