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Product Info Summary
| SKU: | A30468 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-SFRS3 SRSF3 Antibody
SKU/Catalog Number
A30468
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SFRS3 SRSF3 Antibody (Catalog# A30468). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SFRS3 SRSF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30468)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human SFRS3. Immunogen sequence location: 111-160
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30468 is reactive to SRSF3 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
19330 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30468 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from HeLa and Jurkat cells, using SFRS3 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of MCF7 cells, using SFRS3 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For SRSF3 (Source: Uniprot.org, NCBI)
Gene Name
SRSF3
Full Name
Serine/arginine-rich splicing factor 3
Weight
19330 MW
Superfamily
splicing factor SR family
Alternative Names
SRSF3;SFRS3;SRP20;Serine/arginine-rich splicing factor 3;Pre-mRNA-splicing factor SRP20;Splicing factor; arginine/serine-rich 3 SRSF3 SFRS3, SRp20 serine and arginine rich splicing factor 3 serine/arginine-rich splicing factor 3|epididymis secretory sperm binding protein|pre-mRNA splicing factor SRp20|pre-mRNA-splicing factor SRP20|splicing factor, arginine/serine-rich 3|splicing factor, arginine/serine-rich, 20-kD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SRSF3, check out the SRSF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SRSF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SFRS3 SRSF3 Antibody (A30468)
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Customer Q&As
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16 Customer Q&As for Anti-SFRS3 SRSF3 Antibody
Question
My question regarding product A30468, anti-SFRS3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30468 anti-SFRS3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-01
Question
Does A30468 anti-SFRS3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
As indicated on the product datasheet, A30468 anti-SFRS3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-29
Question
Is a blocking peptide available for product anti-SFRS3 antibody (A30468)?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
We do provide the blocking peptide for product anti-SFRS3 antibody (A30468). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-14
Question
See attached the WB image, lot number and protocol we used for brain placenta using anti-SFRS3 antibody A30468. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-03
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain placenta using anti-SFRS3 antibody A30468. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-28
Question
We are currently using anti-SFRS3 antibody A30468 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The anti-SFRS3 antibody (A30468) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-13
Question
I see that the anti-SFRS3 antibody A30468 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-04
Question
I am looking for using your anti-SFRS3 antibody for via spliceosome studies. Has this antibody been tested with western blotting on mcf7 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
We appreciate your inquiry. This A30468 anti-SFRS3 antibody is tested on jurkat cells, mcf7 cells. It is guaranteed to work for IF, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-20
Question
I was wanting to use your anti-SFRS3 antibody for WB for mouse brain placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain placenta identification?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
You can see on the product datasheet, A30468 anti-SFRS3 antibody has been validated for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-23
Question
Does anti-SFRS3 antibody A30468 work for WB with brain placenta?
Verified Customer
Verified customer
Asked: 2018-10-24
Answer
According to the expression profile of brain placenta, SRSF3 is highly expressed in brain placenta. So, it is likely that anti-SFRS3 antibody A30468 will work for WB with brain placenta.
Boster Scientific Support
Answered: 2018-10-24
Question
Do you have a BSA free version of anti-SFRS3 antibody A30468 available?
W. Dhar
Verified customer
Asked: 2018-06-20
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SFRS3 antibody A30468 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-20
Question
Our lab used your anti-SFRS3 antibody for WB on trachea in the past. I am using mouse, and We intend to use the antibody for IF next. I would like examining trachea as well as testis in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2017-09-04
Answer
I have checked the website and datasheets of our anti-SFRS3 antibody and it appears that A30468 has been validated on mouse in both WB and IF. Thus A30468 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-09-04
Question
We are interested in to test anti-SFRS3 antibody A30468 on mouse brain placenta for research purposes, then I may be interested in using anti-SFRS3 antibody A30468 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-11
Answer
The products we sell, including anti-SFRS3 antibody A30468, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-11
Question
Is this A30468 anti-SFRS3 antibody reactive to the isotypes of SRSF3?
P. Mitchell
Verified customer
Asked: 2016-09-22
Answer
The immunogen of A30468 anti-SFRS3 antibody is A peptide derived from human SFRS3. Immunogen sequence location: 111-160. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-09-22
Question
We have been able to see staining in human cervix carcinoma. Are there any suggestions? Is anti-SFRS3 antibody supposed to stain cervix carcinoma positively?
T. Baker
Verified customer
Asked: 2016-03-31
Answer
From literature cervix carcinoma does express SRSF3. From Uniprot.org, SRSF3 is expressed in testis, trachea, brain placenta, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have SRSF3 expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-03-31
Question
My boss were satisfied with the WB result of your anti-SFRS3 antibody. However we have been able to see positive staining in brain placenta nucleus. using this antibody. Is that expected? Could you tell me where is SRSF3 supposed to be expressed?
L. Krishna
Verified customer
Asked: 2013-05-13
Answer
From literature, brain placenta does express SRSF3. Generally SRSF3 expresses in nucleus. Regarding which tissues have SRSF3 expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-05-13
