Anti-SRSF3 Antibody

Product Name

Anti-SRSF3 Antibody

View all SFRS3 Antibodies

SKU/Catalog Number

A03127

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SRSF3 Antibody (Catalog # A03127). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SRSF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-SRSF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03127)

Host

Rabbit

Contents

SRSF3 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SRSF3 antibody was raised against a 19 amino acid peptide near the center of human SRSF3. The immunogen is located within amino acids 80 - 130 of SRSF3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

SRSF3 antibody is human, mouse and rat reactive.

Reactive Species

A03127 is reactive to SRSF3 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

19330 MW

Background of SFRS3

SRSF3, a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors which constitute part of the spliceosome, may be involved in RNA processing in relation with cellular proliferation and/or maturation (1,2). Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins (3). The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation (3,4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03127 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SRSF3 antibody can be used for detection of SRSF3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For SRSF3 (Source: Uniprot.org, NCBI)

Alternative Names

Serine/arginine-rich splicing factor 3, Pre-mRNA-splicing factor SRP20, SFRS3, Splicing factor arginine/serine-rich 3, SRP20 SRSF3 SFRS3, SRp20 serine and arginine rich splicing factor 3 serine/arginine-rich splicing factor 3|epididymis secretory sperm binding protein|pre-mRNA splicing factor SRp20|pre-mRNA-splicing factor SRP20|splicing factor, arginine/serine-rich 3|splicing factor, arginine/serine-rich, 20-kD

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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