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Product Info Summary
| SKU: | PB9509 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-SFRS3/SRSF3 Antibody Picoband®
SKU/Catalog Number
PB9509
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SFRS3/SRSF3 Antibody Picoband® catalog # PB9509. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SFRS3/SRSF3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9509)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SFRS3, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9509 is reactive to SRSF3 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
19 kDa
Calculated molecular weight
19330 MW
Background of SFRS3
Splicing factor, arginine/serine-rich 3, also known as SRSF3, is a protein that in humans is encoded by the SFRS3 gene. The protein encoded by this gene is a member of the serine/ arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9509 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human SH-SY5Y whole cell, human 293T whole cell, human HepG2 whole cell, human SiHa whole cell
ICC/IF: A549 cell
FCM: SH-SY5Y cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SFRS3 using anti-SFRS3 antibody (PB9509).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human SiHa whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRS3 antigen affinity purified polyclonal antibody (Catalog # PB9509) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRS3 at approximately 19 kDa. The expected band size for SFRS3 is at 19 kDa.
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Figure 2. IF analysis of SFRS3 using anti-SFRS3 antibody (PB9509) and anti-Beta Tubulin antibody (M01857-3).
SFRS3 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SFRS3 Antibody (PB9509) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of SH-SY5Y cells using anti-SFRS3 antibody (PB9509).
Overlay histogram showing SH-SY5Y cells stained with PB9509 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFRS3 Antibody (PB9509, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SRSF3 (Source: Uniprot.org, NCBI)
Gene Name
SRSF3
Full Name
Serine/arginine-rich splicing factor 3
Weight
19330 MW
Superfamily
splicing factor SR family
Alternative Names
Serine/arginine-rich splicing factor 3;Pre-mRNA-splicing factor SRP20;Splicing factor, arginine/serine-rich 3;SRSF3;SFRS3, SRP20; SRSF3 SFRS3, SRp20 serine and arginine rich splicing factor 3 serine/arginine-rich splicing factor 3|epididymis secretory sperm binding protein|pre-mRNA splicing factor SRp20|pre-mRNA-splicing factor SRP20|splicing factor, arginine/serine-rich 3|splicing factor, arginine/serine-rich, 20-kD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SRSF3, check out the SRSF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SRSF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SFRS3/SRSF3 Antibody Picoband® (PB9509)
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Customer Q&As
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4 Customer Q&As for Anti-SFRS3/SRSF3 Antibody Picoband®
Question
My question regards using your anti-SFRS3/SRSF3 antibody for regulation of mrna splicing studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
Thanks for your inquiry. This PB9509 anti-SFRS3/SRSF3 antibody is tested on rat brain tissue, tissue lysate, mouse brain, hela whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-05-01
Question
My team were happy with the WB result of your anti-SFRS3/SRSF3 antibody. However we have seen positive staining in brain placenta nucleus. using this antibody. Is that expected? Could you tell me where is SRSF3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
From literature, brain placenta does express SRSF3. Generally SRSF3 expresses in nucleus. Regarding which tissues have SRSF3 expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-22
Question
We are currently using anti-SFRS3/SRSF3 antibody PB9509 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
M. Zhang
Verified customer
Asked: 2018-07-26
Answer
The anti-SFRS3/SRSF3 antibody (PB9509) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-26
Question
We have seen staining in human trachea. What should we do? Is anti-SFRS3/SRSF3 antibody supposed to stain trachea positively?
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
According to literature trachea does express SRSF3. According to Uniprot.org, SRSF3 is expressed in testis, trachea, brain placenta, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have SRSF3 expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-12-25
