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Product Info Summary
| SKU: | A06414-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SF3B4 Antibody Picoband®
SKU/Catalog Number
A06414-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SF3B4 Antibody Picoband® catalog # A06414-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SF3B4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06414-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SF3B4, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06414-1 is reactive to SF3B4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
44 kDa
Calculated molecular weight
26056 MW
Background of SF3B4
Splicing factor 3B subunit 4 is a protein that in humans is encoded by the SF3B4 gene. This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06414-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human MCF-7 whole cell, human U2OS whole cell, human HGC-27 whole cell, human GES-1 whole cell, rat brain tissue, rat stomach tissue, mouse brain tissue, mouse stomach tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SF3B4 using anti-SF3B4 antibody (A06414-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human HGC-27 whole cell lysates,
Lane 4: human GES-1 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat stomach tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3B4 antigen affinity purified polyclonal antibody (Catalog # A06414-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3B4 at approximately 44 kDa. The expected band size for SF3B4 is at 44 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SF3B4 (Source: Uniprot.org, NCBI)
Gene Name
SF3B4
Full Name
Splicing factor 3B subunit 4
Weight
26056 MW
Superfamily
SF3B4 family
Alternative Names
Splicing factor 3B subunit 4; Pre-mRNA-splicing factor SF3b 49 kDa subunit; Spliceosome-associated protein 49; SAP 49; SF3B4; SAP49 SF3B4 AFD1, Hsh49, SAP49, SF3b49 splicing factor 3b subunit 4 splicing factor 3B subunit 4|SAP 49|SF3b50|pre-mRNA-splicing factor SF3b 49 kDa subunit|spliceosomal protein|spliceosome-associated protein (U2 snRNP)|spliceosome-associated protein 49|splicing factor 3b, subunit 4, 49kD|splicing factor 3b, subunit 4, 49kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SF3B4, check out the SF3B4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SF3B4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SF3B4 Antibody Picoband® (A06414-1)
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1 Customer Q&As for Anti-SF3B4 Antibody Picoband®
Question
We are currently using anti-SF3B4 antibody A06414-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-28
Answer
The anti-SF3B4 antibody (A06414-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-28
