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Product Info Summary
| SKU: | M06414 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Splicing Factor SF3B4 Monoclonal Antibody
SKU/Catalog Number
M06414
Size
100ul
Description
Boster Bio Anti-Splicing Factor SF3B4 Monoclonal Antibody catalog # M06414. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Splicing Factor SF3B4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06414)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Isotype
IgG2b
Immunogen
Full length recombinant human SF3B4 expressed in and purified from E. coli
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06414 is reactive to SF3B4 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
44386 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06414 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000. IF/ICC and IHC: 1:1,000
Validation Images & Assay Conditions
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Western blot analysis of different cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to splicing factor SF3B4, M06414, dilution 1:1,000 in green: [1] protein standard (red), [2] NIH-3T3 cytosolic fraction [3] NIH-3T3 nuclear fraction [4] HeLa cytosolic and [5] HeLa nuclear fractions. Strong single band at 49kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with rabbit pAb to GAPDH, dilution 1:20,000, in red. The 37kDa band corresponds to the GAPDH protein, detected mainly in the cytosolic fractions of these cells.
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Immunofluorescent analysis of HeLa cells stained with mouse mAb to splicing factor SF3B4, M06414, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The M06414 antibody reveals strong granular
Protein Target Info & Infographic
Gene/Protein Information For SF3B4 (Source: Uniprot.org, NCBI)
Gene Name
SF3B4
Full Name
Splicing factor 3B subunit 4
Weight
44386 MW
Superfamily
SF3B4 family
Alternative Names
Splicing factor 3B subunit 4;Pre-mRNA-splicing factor SF3b 49 kDa subunit;Spliceosome-associated protein 49;SAP 49;SF3B4;SAP49; SF3B4 AFD1, Hsh49, SAP49, SF3b49 splicing factor 3b subunit 4 splicing factor 3B subunit 4|SAP 49|SF3b50|pre-mRNA-splicing factor SF3b 49 kDa subunit|spliceosomal protein|spliceosome-associated protein (U2 snRNP)|spliceosome-associated protein 49|splicing factor 3b, subunit 4, 49kD|splicing factor 3b, subunit 4, 49kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SF3B4, check out the SF3B4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SF3B4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Splicing Factor SF3B4 Monoclonal Antibody (M06414)
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No publications found for M06414
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Customer Q&As
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7 Customer Q&As for Anti-Splicing Factor SF3B4 Monoclonal Antibody
Question
Would anti-Splicing Factor SF3B4 Monoclonal antibody M06414 work on dog IHC with skin testis?
W. Williams
Verified customer
Asked: 2020-01-22
Answer
Our lab technicians have not validated anti-Splicing Factor SF3B4 Monoclonal antibody M06414 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Splicing Factor SF3B4 Monoclonal antibody M06414 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog skin testis in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-22
Question
I see that the anti-Splicing Factor SF3B4 Monoclonal antibody M06414 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-10
Question
Our lab want to know about to test anti-Splicing Factor SF3B4 Monoclonal antibody M06414 on mouse skin testis for research purposes, then I may be interested in using anti-Splicing Factor SF3B4 Monoclonal antibody M06414 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
The products we sell, including anti-Splicing Factor SF3B4 Monoclonal antibody M06414, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-06
Question
Will M06414 anti-Splicing Factor SF3B4 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
It shows on the product datasheet, M06414 anti-Splicing Factor SF3B4 Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-15
Question
Would anti-Splicing Factor SF3B4 Monoclonal antibody M06414 work for IHC with skin testis?
Verified Customer
Verified customer
Asked: 2019-02-22
Answer
According to the expression profile of skin testis, SF3B4 is highly expressed in skin testis. So, it is likely that anti-Splicing Factor SF3B4 Monoclonal antibody M06414 will work for IHC with skin testis.
Boster Scientific Support
Answered: 2019-02-22
Question
Is this M06414 anti-Splicing Factor SF3B4 Monoclonal antibody reactive to the isotypes of SF3B4?
K. Anderson
Verified customer
Asked: 2017-05-31
Answer
The immunogen of M06414 anti-Splicing Factor SF3B4 Monoclonal antibody is Full length recombinant human SF3B4 expressed in and purified from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-05-31
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin testis using anti-Splicing Factor SF3B4 Monoclonal antibody M06414. Let me know if you need anything else.
T. Huang
Verified customer
Asked: 2016-03-28
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-03-28
