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Product Info Summary
| SKU: | PA1912 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Delta-like protein 3 DLL3 Antibody Picoband®
SKU/Catalog Number
PA1912
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Delta-like protein 3 DLL3 Antibody catalog # PA1912. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Delta-like protein 3 DLL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1912)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human DLL3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1912 is reactive to DLL3 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
64618 MW
Background of DLL3
DLL3 (DELTA-LIKE 3) also known as DELTA, DROSOPHILA, HOMOLOG OF, is a protein which in humans is encoded by the DLL3 gene. This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutation in the mouse delta-like 3 gene (Dll3), which is homologous to the Notch-ligand delta in Drosophila, results in the mouse 'pudgy' phenotype. The human DLL3 gene was identified within a critical interval, mapped in 2 consanguineous Arab-Israeli and Pakistani SCDO1 pedigrees, of 7.8 cM at 19q13.1-q13.3 between D19S570 and D19S908 (Bulman et al., 2000). Dunwoodie et al. (1997) presented results suggesting that mouse Dll3 may complement the function of other delta homologs during early pattern formation in the mouse embryo. In humans, the fact that mutations in genes required for oscillation, such as DLL3, result in abnormal segmentation of the vertebral column suggests that the segmentation clock also acts during human embryonic development. This residue is highly conserved in Delta proteins from Drosophila to humans, and the substitution of a charged polar for a nonpolar residue may disrupt the conformation of the DLL3 protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1912 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: rat brain tissue, mouse brain tissue, human HEK293 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DLL3 using anti-DLL3 antibody (PA1912).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates
Lane 2: mouse brain tissue lysates
Lane 3: human HEK293 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL3 antigen affinity purified polyclonal antibody (Catalog # PA1912) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL3 at approximately 65KD. The expected band size for DLL3 is at 65KD.
Protein Target Info & Infographic
Gene/Protein Information For DLL3 (Source: Uniprot.org, NCBI)
Gene Name
DLL3
Full Name
Delta-like protein 3
Weight
64618 MW
Alternative Names
Delta-like protein 3;Drosophila Delta homolog 3;Delta3;DLL3; DLL3 SCDO1 delta like canonical Notch ligand 3 delta-like protein 3|delta-like 3|delta3|drosophila Delta homolog 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DLL3, check out the DLL3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DLL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Delta-like protein 3 DLL3 Antibody Picoband® (PA1912)
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Customer Q&As
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16 Customer Q&As for Anti-Delta-like protein 3 DLL3 Antibody Picoband®
Question
We have been able to see staining in human brain. What should we do? Is anti-DLL3 antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
Based on literature brain does express DLL3. Based on Uniprot.org, DLL3 is expressed in nucleus accumbens, brain, among other tissues. Regarding which tissues have DLL3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-14
Question
Our team were well pleased with the WB result of your anti-DLL3 antibody. However we have been able to see positive staining in nucleus accumbens membrane using this antibody. Is that expected? Could you tell me where is DLL3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
Based on literature, nucleus accumbens does express DLL3. Generally DLL3 expresses in membrane. Regarding which tissues have DLL3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-10
Question
I was wanting to use your anti-DLL3 antibody for WB for human nucleus accumbens on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human nucleus accumbens identification?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
It shows on the product datasheet, PA1912 anti-DLL3 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human nucleus accumbens in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-27
Question
Will anti-DLL3 antibody PA1912 work for WB with nucleus accumbens?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
According to the expression profile of nucleus accumbens, DLL3 is highly expressed in nucleus accumbens. So, it is likely that anti-DLL3 antibody PA1912 will work for WB with nucleus accumbens.
Boster Scientific Support
Answered: 2020-01-31
Question
I was wanting to use using your anti-DLL3 antibody for notch signaling pathway studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
Thank you for your inquiry. This PA1912 anti-DLL3 antibody is validated on rat brain tissue, mouse brain, human hek293 whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-11
Question
Would PA1912 anti-DLL3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
It shows on the product datasheet, PA1912 anti-DLL3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-16
Question
Is a blocking peptide available for product anti-DLL3 antibody (PA1912)?
H. Walker
Verified customer
Asked: 2019-06-20
Answer
We do provide the blocking peptide for product anti-DLL3 antibody (PA1912). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-20
Question
Would anti-DLL3 antibody PA1912 work on primate WB with nucleus accumbens?
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
Our lab technicians have not validated anti-DLL3 antibody PA1912 on primate. You can run a BLAST between primate and the immunogen sequence of anti-DLL3 antibody PA1912 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate nucleus accumbens in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-28
Question
I see that the anti-DLL3 antibody PA1912 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-12
Question
Do you have a BSA free version of anti-DLL3 antibody PA1912 available?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-DLL3 antibody PA1912 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-09
Question
Is this PA1912 anti-DLL3 antibody reactive to the isotypes of DLL3?
Verified Customer
Verified customer
Asked: 2017-07-31
Answer
The immunogen of PA1912 anti-DLL3 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human DLL3(599-618aa RAGQRQHLLFPYPSSILSVK). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-31
Question
See attached the WB image, lot number and protocol we used for nucleus accumbens using anti-DLL3 antibody PA1912. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-13
Question
I have a question about product PA1912, anti-DLL3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Taylor
Verified customer
Asked: 2017-02-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1912 anti-DLL3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-02-06
Question
We need to test anti-DLL3 antibody PA1912 on human nucleus accumbens for research purposes, then I may be interested in using anti-DLL3 antibody PA1912 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Mangal
Verified customer
Asked: 2015-02-05
Answer
The products we sell, including anti-DLL3 antibody PA1912, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-02-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for nucleus accumbens using anti-DLL3 antibody PA1912. Let me know if you need anything else.
K. Bhatt
Verified customer
Asked: 2013-10-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-10-22
Question
We are currently using anti-DLL3 antibody PA1912 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
M. Yang
Verified customer
Asked: 2013-06-03
Answer
The anti-DLL3 antibody (PA1912) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-03
