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Product Info Summary
| SKU: | A05559-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-SESN1/Pa26 Antibody
SKU/Catalog Number
A05559-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SESN1/Pa26 Antibody (Catalog# A05559-1). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SESN1/Pa26 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05559-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human SESN1. Immunogen sequence location: 271 - 320
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05559-1 is reactive to SESN1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
56557 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05559-1 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from rat muscle cells, using SESN1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SESN1 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For SESN1 (Source: Uniprot.org, NCBI)
Gene Name
SESN1
Full Name
Sestrin-1
Weight
56557 MW
Superfamily
sestrin family
Alternative Names
SESN1;PA26;SEST1;Sestrin-1;p53-regulated protein PA26 SESN1 PA26, SEST1 sestrin 1 sestrin-1|p53 activated gene 26|p53 regulated PA26 nuclear protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SESN1, check out the SESN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SESN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SESN1/Pa26 Antibody (A05559-1)
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No publications found for A05559-1
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SESN1/Pa26 Antibody
Question
Is a blocking peptide available for product anti-SESN1/Pa26 antibody (A05559-1)?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
We do provide the blocking peptide for product anti-SESN1/Pa26 antibody (A05559-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-19
Question
I was wanting to use your anti-SESN1/Pa26 antibody for IHC for human muscle organ on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human muscle organ identification?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
It shows on the product datasheet, A05559-1 anti-SESN1/Pa26 antibody has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human muscle organ in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle organ using anti-SESN1/Pa26 antibody A05559-1. Let me know if you need anything else.
N. Kulkarni
Verified customer
Asked: 2019-06-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-11
Question
Do you have a BSA free version of anti-SESN1/Pa26 antibody A05559-1 available?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SESN1/Pa26 antibody A05559-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-21
Question
We are currently using anti-SESN1/Pa26 antibody A05559-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
The anti-SESN1/Pa26 antibody (A05559-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-07
Question
Does anti-SESN1/Pa26 antibody A05559-1 work for IHC with muscle organ?
A. Wu
Verified customer
Asked: 2017-02-22
Answer
According to the expression profile of muscle organ, SESN1 is highly expressed in muscle organ. So, it is likely that anti-SESN1/Pa26 antibody A05559-1 will work for IHC with muscle organ.
Boster Scientific Support
Answered: 2017-02-22
Question
Is this A05559-1 anti-SESN1/Pa26 antibody reactive to the isotypes of SESN1?
J. Williams
Verified customer
Asked: 2015-06-29
Answer
The immunogen of A05559-1 anti-SESN1/Pa26 antibody is A peptide derived from human SESN1. Immunogen sequence location: 271 - 320. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-06-29
