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Product Info Summary
| SKU: | PA1976 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-53BP1/TP53BP1 Antibody Picoband®
SKU/Catalog Number
PA1976
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-53BP1/TP53BP1 Antibody catalog # PA1976. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-53BP1/TP53BP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1976)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1, identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1976 is reactive to TP53BP1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
450 kDa
Calculated molecular weight
213574 MW
Background of 53BP1
TP53BP1 (Tumor Protein p53-Binding Protein 1), also called 53BP1, is a protein that in humans is encoded by the TP53BP1 gene. Iwabuchi et al. (1998) mapped the TP53BP1 gene to 15q15-q21 by FISH. Iwabuchi et al. (1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro. Immunoblot analysis by Iwabuchi et al. (1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21 (CDKN1A). Wang et al. (2002) used small interfering RNA directed against TP53BP1 in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal. TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1976 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human
Positive Control
WB: human Hela whole cell, human RT4 whole cell
IHC: Human Intestinal Cancer tissue, Rat Brain tissue, Mouse Brain tissue
ICC: A549 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TP53BP1 using anti-TP53BP1 antibody (PA1976).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53BP1 antigen affinity purified polyclonal antibody (Catalog # PA1976) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53BP1 at approximately 450 kDa. The expected band size for TP53BP1 is at 214 kDa.
Click image to see more details
Figure 2. IHC analysis of TP53BP1 using anti-TP53BP1 antibody (PA1976).
TP53BP1 was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TP53BP1 Antibody (PA1976) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of TP53BP1 using anti-TP53BP1 antibody (PA1976).
TP53BP1 was detected in a paraffin-embedded section of Rat Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TP53BP1 Antibody (PA1976) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of TP53BP1 using anti-TP53BP1 antibody (PA1976).
TP53BP1 was detected in a paraffin-embedded section of Mouse Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TP53BP1 Antibody (PA1976) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. ICC analysis of TP53BP1 using anti-TP53BP1 antibody (PA1976).
TP53BP1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-TP53BP1 Antibody (PA1976) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TP53BP1 (Source: Uniprot.org, NCBI)
Gene Name
TP53BP1
Full Name
TP53-binding protein 1
Weight
213574 MW
Alternative Names
Tumor suppressor p53-binding protein 1;53BP1;p53-binding protein 1;p53BP1;TP53BP1; TP53BP1 53BP1, TDRD30, p202, p53BP1 tumor protein p53 binding protein 1 TP53-binding protein 1|p53-binding protein 1|tumor protein 53-binding protein, 1|tumor suppressor p53-binding protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TP53BP1, check out the TP53BP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TP53BP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-53BP1/TP53BP1 Antibody Picoband® (PA1976)
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No publications found for PA1976
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Customer Q&As
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17 Customer Q&As for Anti-53BP1/TP53BP1 Antibody Picoband®
Question
We need to test anti-53BP1/TP53BP1 antibody PA1976 on human cervix for research purposes, then I may be interested in using anti-53BP1/TP53BP1 antibody PA1976 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
The products we sell, including anti-53BP1/TP53BP1 antibody PA1976, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-10
Question
Do you have a BSA free version of anti-53BP1/TP53BP1 antibody PA1976 available?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-53BP1/TP53BP1 antibody PA1976 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-17
Question
We have tried in the past anti-53BP1/TP53BP1 antibody for IHC on cervix carcinoma erythroleukemia in the past. I am using rat, and We want to use the antibody for WB next. I am interested in examining cervix carcinoma erythroleukemia as well as embryonic kidney in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
I looked at the website and datasheets of our anti-53BP1/TP53BP1 antibody and it appears that PA1976 has been validated on rat in both IHC and WB. Thus PA1976 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-25
Question
Can you help my question with product PA1976, anti-53BP1/TP53BP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Parker
Verified customer
Asked: 2019-06-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1976 anti-53BP1/TP53BP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-10
Question
Will PA1976 anti-53BP1/TP53BP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
As indicated on the product datasheet, PA1976 anti-53BP1/TP53BP1 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-31
Question
I was wanting to use your anti-53BP1/TP53BP1 antibody for ICC for human cervix on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix identification?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
You can see on the product datasheet, PA1976 anti-53BP1/TP53BP1 antibody has been validated for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-16
Question
I see that the anti-53BP1/TP53BP1 antibody PA1976 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-22
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-22
Question
Does anti-53BP1/TP53BP1 antibody PA1976 work on bovine WB with skeletal muscle?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
Our lab technicians have not validated anti-53BP1/TP53BP1 antibody PA1976 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-53BP1/TP53BP1 antibody PA1976 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine skeletal muscle in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-06
Question
See below the WB image, lot number and protocol we used for cervix using anti-53BP1/TP53BP1 antibody PA1976. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-25
Question
I would like using your anti-53BP1/TP53BP1 antibody for double-strand break repair via nonhomologous end joining studies. Has this antibody been tested with western blotting on hepa cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-04-26
Answer
Thank you for your inquiry. This PA1976 anti-53BP1/TP53BP1 antibody is tested on 293t cell lysate, hepa cell lysate, intestinal cancer tissue, rat brain tissue, mouse brain. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-04-26
Question
We are currently using anti-53BP1/TP53BP1 antibody PA1976 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
B. Jones
Verified customer
Asked: 2018-04-04
Answer
The anti-53BP1/TP53BP1 antibody (PA1976) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-04
Question
Is this PA1976 anti-53BP1/TP53BP1 antibody reactive to the isotypes of TP53BP1?
Verified Customer
Verified customer
Asked: 2017-10-18
Answer
The immunogen of PA1976 anti-53BP1/TP53BP1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1(1955-1972aa ERIGFKQHPKYKHDYVSH), identical to the related mouse sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-18
Question
We have seen staining in human myeloid leukemia cell. Do you have any suggestions? Is anti-53BP1/TP53BP1 antibody supposed to stain myeloid leukemia cell positively?
Verified Customer
Verified customer
Asked: 2017-09-01
Answer
From what I have seen in literature myeloid leukemia cell does express TP53BP1. From what I have seen in Uniprot.org, TP53BP1 is expressed in lung, skeletal muscle, myeloid leukemia cell, cervix, cerebellum, cervix carcinoma, embryonic kidney, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have TP53BP1 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 15489334
Cervix, Pubmed ID: 17974005
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Skeletal muscle, Pubmed ID: 9748285
Boster Scientific Support
Answered: 2017-09-01
Question
We were satisfied with the WB result of your anti-53BP1/TP53BP1 antibody. However we have seen positive staining in lung nucleus using this antibody. Is that expected? Could you tell me where is TP53BP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-08-29
Answer
Based on literature, lung does express TP53BP1. Generally TP53BP1 expresses in nucleus. Regarding which tissues have TP53BP1 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 15489334
Cervix, Pubmed ID: 17974005
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Skeletal muscle, Pubmed ID: 9748285
Boster Scientific Support
Answered: 2017-08-29
Question
Will anti-53BP1/TP53BP1 antibody PA1976 work for ICC with cervix?
A. Mitchell
Verified customer
Asked: 2016-08-10
Answer
According to the expression profile of cervix, TP53BP1 is highly expressed in cervix. So, it is likely that anti-53BP1/TP53BP1 antibody PA1976 will work for ICC with cervix.
Boster Scientific Support
Answered: 2016-08-10
Question
Is a blocking peptide available for product anti-53BP1/TP53BP1 antibody (PA1976)?
C. Collins
Verified customer
Asked: 2015-10-06
Answer
We do provide the blocking peptide for product anti-53BP1/TP53BP1 antibody (PA1976). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-10-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix using anti-53BP1/TP53BP1 antibody PA1976. Let me know if you need anything else.
B. Miller
Verified customer
Asked: 2015-06-10
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-06-10
