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Product Info Summary
| SKU: | A05088-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SENP6 Picoband® Antibody
SKU/Catalog Number
A05088-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SENP6 Picoband® Antibody catalog # A05088-3. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SENP6 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05088-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SENP6 recombinant protein (Position: R135-K1086).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05088-3 is reactive to SENP6 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
75991 MW
Background of SENP6
Sentrin-specific protease 6 is an enzyme that in humans is encoded by the SENP6 gene. It is mapped to 6q14.1. Ubiquitin-like molecules (UBLs), such as SUMO1, are structurally related to ubiquitin and can be ligated to target proteins in a similar manner as ubiquitin. However, covalent attachment of UBLs does not result in degradation of the modified proteins. SUMO1 modification is implicated in the targeting of RANGAP1 to the nuclear pore complex, as well as in stabilization of I-kappa-B-alpha from degradation by the 26S proteasome. Like ubiquitin, UBLs are synthesized as precursor proteins, with 1 or more amino acids following the C-terminal glycine-glycine residues of the mature UBL protein. Thus, the tail sequences of the UBL precursors need to be removed by UBL-specific proteases, such as SENP6, prior to their conjugation to target proteins. SENPs also display isopeptidase activity for deconjugation of SUMO-conjugated substrates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05088-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human K562 whole cell, human Raji whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SENP6 using anti-SENP6 antibody (A05088-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Raji whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP6 antigen affinity purified polyclonal antibody (Catalog # A05088-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SENP6 at approximately 150KD. The expected band size for SENP6 is at 126KD.
Protein Target Info & Infographic
Gene/Protein Information For SENP6 (Source: Uniprot.org, NCBI)
Gene Name
SENP6
Full Name
Sentrin-specific protease 6
Weight
75991 MW
Superfamily
peptidase C48 family
Alternative Names
Sentrin-specific protease 6; SUMO-1-specific protease 1; Sentrin/SUMO-specific protease SENP6; SENP6; KIAA0797; SSP1; SUSP1; FKSG6 SENP6 SSP1, SUSP1 SUMO specific peptidase 6 sentrin-specific protease 6|2810017C20Rik|SUMO-1-specific protease 1|SUMO1/sentrin specific peptidase 6|SUMO1/sentrin specific protease 6|sentrin/SUMO-specific protease SENP6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SENP6, check out the SENP6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SENP6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SENP6 Picoband® Antibody (A05088-3)
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