Key=store_id Key=entity_id, value=45437 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=A01725 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2018-01-31 04:47:58 Key=updated_at, value=2024-09-26 00:57:25 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-SEK1/MKK4 (Ab-261) MAP2K4 Antibody Key=cj_search_index Key=publication_count, value=0 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Rabbit Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1211 Key=yoast_content_score, value=60 Key=price, value=360.0000 Key=weight, value=1.0000 Key=meta_title, value=Anti-SEK1/MKK4 (Ab-261) Antibody | IHC, IF Validated | Bosterbio Key=meta_description, value=Boster Bio Anti-SEK1/MKK4 (Ab-261) MAP2K4 Antibody (Catalog # A01725). Tested in IHC, IF applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μl in Liquid form antibody. Key=image, value=/A/0/A01725-MAP2K4-IF-test-2.jpg Key=small_image, value=/A/0/A01725-MAP2K4-IF-test-2.jpg Key=thumbnail, value=/A/0/A01725-MAP2K4-IF-test-2.jpg Key=url_key, value=anti-sek1-mkk4-ab-261-antibody-a01725-boster Key=url_path, value=anti-sek1-mkk4-ab-261-antibody-a01725-boster Key=options_container, value=Product Info Column Key=image_label, value=Immunofluorescence staining of methanol-fixed Hela cells using SEK1/MKK4(Ab-261) Antibody #A01725. Key=small_image_label, value=Immunofluorescence staining of methanol-fixed Hela cells using SEK1/MKK4(Ab-261) Antibody #A01725. Key=thumbnail_label, value=Immunofluorescence staining of methanol-fixed Hela cells using SEK1/MKK4(Ab-261) Antibody #A01725. Key=msrp_display_actual_price_type, value= Key=gift_message_available, value=No Key=size, value=100 µl Key=protein_name, value=CD99 antigen Key=gene_full_name, value=CD99 antigen Key=uniprot_id, value=P45985 Key=applications, value=IF, IHC Key=reactivity, value=Human, Mouse, Rat Key=form, value=Liquid Key=concentration, value=1 mg/ml Key=predicted_reactivity, value=Bovine, Canine, Equine, Guinea Pig, Pig, Rabbit, Sheep Key=source_company, value=SabBiotech 21131; Cost: 159.5 Key=isotype, value=IgG Key=molecular_weight, value=18848 MW Key=related_product_skus, value=A01718,PROTP05187 Key=product_category, value=Primary Antibodies Key=research_category, value=MAPK Pathway, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction Key=clonality, value=Polyclonal Key=swatch_image, value=/a/0/a01393-1-western-blotting-analysis-1_1.jpg Key=rating_value, value=100 Key=review_count, value=5 Key=mp_exclude_sitemap, value=No Key=yoast_focus_keyword, value=Cathepsin D/CTSD Antibody Key=yoast_facebook_image, value=/antibody/pb9999-1-WB-anti-cxcl13-blc-picoband-antibody.jpg Key=yoast_twitter_image, value=/antibody/pb9999-1-WB-anti-cxcl13-blc-picoband-antibody.jpg Key=cj_related_products, value=Liver Development:A00804-1|FEK1374|PROTQ9Y5C1-4|PROTQ15771^Protein Kinase:A16582^Tyrosine Kinases:A01989-1 Key=out_of_stock_until_date, value=Oct 20, 2022 Key=description, value=Boster Bio Anti-SEK1/MKK4 (Ab-261) MAP2K4 Antibody (Catalog # A01725). Tested in IHC, IF applications. This antibody reacts with Human, Mouse, Rat. Key=short_description, value=Boster Bio Anti-SEK1/MKK4 (Ab-261) MAP2K4 Antibody (Catalog # A01725). Tested in IHC, IF applications. This antibody reacts with Human, Mouse, Rat. Key=meta_keyword, value=Anti-SEK1/MKK4 (Ab-261) MAP2K4 Antibody Key=contents, value=Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Key=background, value=Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
Wang W, et al. (2002) Mol Cell Biol; 22(10): 3389-403.
Leung IW, et al. (2001)J Biol Chem; 276(3): 1961-7.
Key=gene_name, value=MAP2K4 Key=synonyms, value=CD99 antigen;12E7;E2 antigen;Protein MIC2;T-cell surface glycoprotein E2;CD99;CD99;MIC2, MIC2X, MIC2Y;
Key=application_details, value=Predicted MW: 44kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200 Key=subcellular_localization, value=Membrane ; Single-pass type I
membrane protein .
Key=tissue_specificity, value=Detected in placenta (at protein level).
Key=protein_function, value=Involved in T-cell adhesion processes and in spontaneous
rosette formation with erythrocytes. Plays a role in a late step
of leukocyte extravasation helping leukocytes to overcome the
endothelial basement membrane. Acts at the same site as, but
independently of, PECAM1. Involved in T-cell adhesion processes
(By similarity). .
Key=competitor_equivalent_skus, value=sc 69436|sc 69435|sc 376605|sc 98603|sc 50297|sc 10368 Key=immunogen, value=Peptide sequence around aa.259~263 (A-K-T-R-D) derived from Human SEK1/MKK4. Key=purification, value=Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. Key=storage, value=Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. Key=custom_attribute_1, value=39 kDa Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=quantity_and_stock_status Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-SEK1/MKK4 (Ab-261) MAP2K4 Antibody
MKK4/MEK4 antibody
Boster Bio Anti-SEK1/MKK4 (Ab-261) MAP2K4 Antibody (Catalog # A01725). Tested in IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Boster Bio Anti-SEK1/MKK4 (Ab-261) MAP2K4 Antibody (Catalog # A01725). Tested in IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SEK1/MKK4 (Ab-261) MAP2K4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01725)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.259~263 (A-K-T-R-D) derived from Human SEK1/MKK4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01725 is reactive to MAP2K4 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
18848 MW
Background of MKK4/MEK4
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
Wang W, et al. (2002) Mol Cell Biol; 22(10): 3389-403.
Leung IW, et al. (2001)J Biol Chem; 276(3): 1961-7.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Figure 1. IHC analysis of MAP2K4 using anti-MAP2K4 antibody (A01725). MAP2K4 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP2K4 Antibody (A01725) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using SEK1/MKK4(Ab-261) Antibody #A01725.
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on MAP2K4, check out the MAP2K4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00
Troubleshooting
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