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Product Info Summary
| SKU: | A01725 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC |
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Product info
Product Name
Anti-SEK1/MKK4 (Ab-261) MAP2K4 Antibody
SKU/Catalog Number
A01725
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SEK1/MKK4 (Ab-261) MAP2K4 Antibody (Catalog # A01725). Tested in IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SEK1/MKK4 (Ab-261) MAP2K4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01725)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.259~263 (A-K-T-R-D) derived from Human SEK1/MKK4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01725 is reactive to MAP2K4 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
18848 MW
Background of MKK4/MEK4
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
Wang W, et al. (2002) Mol Cell Biol; 22(10): 3389-403.
Leung IW, et al. (2001)J Biol Chem; 276(3): 1961-7.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01725 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 44kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. IHC analysis of MAP2K4 using anti-MAP2K4 antibody (A01725).
MAP2K4 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP2K4 Antibody (A01725) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Immunofluorescence staining of methanol-fixed Hela cells using SEK1/MKK4(Ab-261) Antibody #A01725.
Protein Target Info & Infographic
Gene/Protein Information For MAP2K4 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K4
Full Name
Dual specificity mitogen-activated protein kinase kinase 4
Weight
18848 MW
Superfamily
protein kinase superfamily
Alternative Names
CD99 antigen;12E7;E2 antigen;Protein MIC2;T-cell surface glycoprotein E2;CD99;CD99;MIC2, MIC2X, MIC2Y; MAP2K4 JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1 mitogen-activated protein kinase kinase 4 dual specificity mitogen-activated protein kinase kinase 4|JNK-activated kinase 1|JNK-activating kinase 1|MAP kinase kinase 4|MAPK/ERK kinase 4|MAPKK 4|MEK 4|SAPK/ERK kinase 1|c-Jun N-terminal kinase kinase 1|stress-activated protein kinase kinase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K4, check out the MAP2K4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SEK1/MKK4 (Ab-261) MAP2K4 Antibody (A01725)
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No publications found for A01725
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