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Product Info Summary
| SKU: | PA2275 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MEK4/MAP2K4 Antibody Picoband®
SKU/Catalog Number
PA2275
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MEK4/MAP2K4 Antibody catalog # PA2275. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEK4/MAP2K4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2275)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MEK4, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2275 is reactive to MAP2K4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
44 kDa
Calculated molecular weight
44288 MW
Background of MKK4/MEK4
Dual specificity mitogen-activated protein kinase kinase 4 (MAP2K4), also called SEK1 or JNKK1, is an enzyme that in humans is encoded by the MAP2K4 gene. It is mapped to 17p12. This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK1. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. MAP2K4 was a specific activator of JNK1, JNK2, and p38, and it has been shown to interact with FLNC, MAPK8, MAPK8IP3 and AKT1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2275 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Skeletal Muscle Tissue, HELA Whole Cell, A549 Whole Cell, MM231 Whole Cell, CEM Whole Cell
Validation Images & Assay Conditions
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Anti-MEK4 antibody, PA2275, All Western blotting
All lanes: Anti-MAP2K4(PA2275) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: CEM Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
Protein Target Info & Infographic
Gene/Protein Information For MAP2K4 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K4
Full Name
Dual specificity mitogen-activated protein kinase kinase 4
Weight
44288 MW
Superfamily
protein kinase superfamily
Alternative Names
Dual specificity mitogen-activated protein kinase kinase 4;MAP kinase kinase 4;MAPKK 4;2.7.12.2;JNK-activating kinase 1;MAPK/ERK kinase 4;MEK 4;SAPK/ERK kinase 1;SEK1;Stress-activated protein kinase kinase 1;SAPK kinase 1;SAPKK-1;SAPKK1;c-Jun N-terminal kinase kinase 1;JNKK;MAP2K4;JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1; MAP2K4 JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1 mitogen-activated protein kinase kinase 4 dual specificity mitogen-activated protein kinase kinase 4|JNK-activated kinase 1|JNK-activating kinase 1|MAP kinase kinase 4|MAPK/ERK kinase 4|MAPKK 4|MEK 4|SAPK/ERK kinase 1|c-Jun N-terminal kinase kinase 1|stress-activated protein kinase kinase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K4, check out the MAP2K4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEK4/MAP2K4 Antibody Picoband® (PA2275)
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No publications found for PA2275
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-MEK4/MAP2K4 Antibody Picoband®
Question
I see that the anti-MEK4/MAP2K4 antibody PA2275 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-05-04
Question
We are currently using anti-MEK4/MAP2K4 antibody PA2275 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-12
Answer
The anti-MEK4/MAP2K4 antibody (PA2275) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-12
Question
Is a blocking peptide available for product anti-MEK4/MAP2K4 antibody (PA2275)?
K. Brown
Verified customer
Asked: 2017-07-24
Answer
We do provide the blocking peptide for product anti-MEK4/MAP2K4 antibody (PA2275). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-07-24
