Anti-SDHA Antibody Picoband™

Product Name

Anti-SDHA Antibody Picoband™

View all SDHA Antibodies

SKU/Catalog Number

PB9433

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SDHA Antibody Picoband™ catalog # PB9433. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SDHA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9433)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SDHA recombinant protein (Position: S44-L380). Human SDHA shares 98.2% and 97.6% amino acid (aa) sequence identity with mouse and rat SDHA, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9433 is reactive to SDHA in Human, Mouse, Rat

Applications

PB9433 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

73 kDa

Calculated molecular weight

72692 MW

Background of SDHA

Complex II of the mitochondrial respiratory chain, also known as succinate dehydrogenase or succinate:ubiquinone oxidoreductase, consists of 4 nuclear-encoded polypeptides, these are the flavoprotein subunit (SDHA), the iron sulfur protein subunit (SDHB), and the integral membrane protein subunits SDHC and SDHD. SDHA is an acronym for succinate dehydrogenase complex subunit A. The succinate dehydrogenase (SDH) protein complex catalyzes the oxidation of succinate (succinate + ubiquinone => fumarate + ubiquinol). The SDHA subunit is connected to the SDHB subunit on the hydrophilic, catalytic end of the complex, and weighs 72.7 kDA. Mutations in the SDHA subunit have a distinct pathology from mutations in the SDHB/SDHC/SDHD subunits; it is the only subunit to never have shown tumor suppressor behaviour. Heterozygous carriers of an SDHA mutation do not develop paragangliomas as has been seen for mutations in the other subunits. This appears to be due to the expression of two similar SDHA genes (Types I and II) in the paraganglia system.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SDHA using anti-SDHA antibody (PB9433).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Liver Tissue Lysate at 50ug,
Lane 2: Mouse Liver Tissue Lysate at 50ug,
Lane 3: Rat Brain Tissue Lysate at 50ug,
Lane 4: Mouse Brain Tissue Lysate at 50ug,
Lane 5: SMMC Whole Cell Lysate at 40ug,
Lane 6: Human Placenta Tissue Lysate at 50ug,
Lane 7: SW620 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDHA antigen affinity purified polyclonal antibody (Catalog # PB9433) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDHA at approximately 73 kDa. The expected band size for SDHA is at 73 kDa.

Gene/Protein Information For SDHA (Source: Uniprot.org, NCBI)

Alternative Names

CMD1GG; EC 1.3.5.1; Fp; SDH1; SDH2; SDHFmitochondrial; succinate dehydrogenase complex flavoprotein subunit; succinate dehydrogenase complex, subunit A, flavoprotein (Fp) SDHA CMD1GG, FP, MC2DN1, PGL5, SDH1, SDH2, SDHF succinate dehydrogenase complex flavoprotein subunit A succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial|flavoprotein subunit of complex II|succinate dehydrogenase complex, subunit A, flavoprotein (Fp)

For more info on SDHA, check out the SDHA Infographic

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In this infographic, you will see the following information for SDHA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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