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Product Info Summary
| SKU: | M01753 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SDHA Rabbit Monoclonal Antibody
SKU/Catalog Number
M01753
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SDHA Rabbit Monoclonal Antibody catalog # M01753. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SDHA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01753)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AADA-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SDHA
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01753 is reactive to SDHA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
73 kDa
Calculated molecular weight
72692 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01753 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
FC 1:20
Positive Control
WB: human Hela whole cell, human PC-3 whole cell, human 293T whole cell, human HepG2 whole cell, rat brain tissue, rat kidney tissue, mouse brain tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SDHA using anti-SDHA antibody (M01753).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDHA antigen affinity purified monoclonal antibody (Catalog # M01753) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDHA at approximately 73 kDa. The expected band size for SDHA is at 73 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SDHA (Source: Uniprot.org, NCBI)
Gene Name
SDHA
Full Name
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
Weight
72692 MW
Superfamily
FAD-dependent oxidoreductase 2 family
Alternative Names
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial;1.3.5.1;Flavoprotein subunit of complex II;Fp;SDHA;SDH2, SDHF; SDHA CMD1GG, FP, MC2DN1, PGL5, SDH1, SDH2, SDHF succinate dehydrogenase complex flavoprotein subunit A succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial|flavoprotein subunit of complex II|succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SDHA, check out the SDHA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SDHA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SDHA Rabbit Monoclonal Antibody (M01753)
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