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Product Info Summary
| SKU: | A01461-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-BTG2 Antibody Picoband®
SKU/Catalog Number
A01461-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BTG2 Antibody Picoband® catalog # A01461-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BTG2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01461-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human BTG2, which shares 88.9% amino acid (aa) sequence identity with both mouse and rat BTG2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01461-1 is reactive to BTG2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
43363 MW
Background of BTG2
Protein BTG2, also known as BTG family member 2 or NGF-inducible anti-proliferative protein PC3 or NGF-inducible protein TIS21, is a protein that in humans is encoded by the BTG2 gene. The protein encoded by the gene BTG2 is a member of the BTG/Tob family. This protein controls cell cycle progression and proneural genes expression by acting as a transcription coregulator that enhances or inhibits the activity of transcription factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01461-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
WB: human HepG2 whole cell, human PC-3 whole cell, human U20S whole cell, human A549 whole cell
FCM: A431 cell
Validation Images & Assay Conditions
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Figure 2. Flow Cytometry analysis of A431 cells using anti-BTG2 antibody (A01461-1).
Overlay histogram showing A431 cells stained with A01461-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BTG2 Antibody (A01461-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of BTG2 using anti-BTG2 antibody (A01461-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTG2 antigen affinity purified polyclonal antibody (Catalog # A01461-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BTG2 at approximately 22 kDa. The expected band size for BTG2 is at 17 kDa.
Protein Target Info & Infographic
Gene/Protein Information For BTG2 (Source: Uniprot.org, NCBI)
Gene Name
BTG2
Full Name
Protein BTG2
Weight
43363 MW
Superfamily
BTG family
Alternative Names
Protein BTG2; BTG family member 2; NGF-inducible anti-proliferative protein PC3; BTG2; PC3 BTG2 APRO1, PC3, TIS21 BTG anti-proliferation factor 2 protein BTG2|B-cell translocation gene 2|BTG family member 2|NGF-inducible anti-proliferative protein PC3|nerve growth factor-inducible anti-proliferative|pheochromacytoma cell-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BTG2, check out the BTG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BTG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BTG2 Antibody Picoband® (A01461-1)
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Customer Q&As
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4 Customer Q&As for Anti-BTG2 Antibody Picoband®
Question
We are currently using anti-BTG2 antibody A01461-1 for mouse tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
C. Zhao
Verified customer
Asked: 2019-11-07
Answer
The anti-BTG2 antibody (A01461-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-07
Question
you antibody to test anti-BTG2 antibody A01461-1 on rat fetal liver for research purposes, then I may be interested in using anti-BTG2 antibody A01461-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
The products we sell, including anti-BTG2 antibody A01461-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-03
Question
Would anti-BTG2 antibody A01461-1 work on horse WB with fetal liver?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
Our lab technicians have not tested anti-BTG2 antibody A01461-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-BTG2 antibody A01461-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse fetal liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-12
Question
Will A01461-1 anti-BTG2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Evans
Verified customer
Asked: 2017-07-06
Answer
It shows on the product datasheet, A01461-1 anti-BTG2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-06
