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Product Info Summary
| SKU: | M07463 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SCIN Rabbit Monoclonal Antibody
SKU/Catalog Number
M07463
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-SCIN Rabbit Monoclonal Antibody catalog # M07463. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SCIN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07463)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
27S73
Isotype
IgG
Immunogen
A synthesized peptide derived from human SCIN
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07463 is reactive to SCIN in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
80 kDa
Calculated molecular weight
80 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07463 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: rat kidney tissue, mouse kidney tissue, mouse spleen tissue, human 293 whole cell, human SH-SY5Y whole cell, human A673 whole cell,
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SCIN using anti-SCIN antibody (M07463).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates,
Lane 3: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCIN antigen affinity purified monoclonal antibody (M07463) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCIN at approximately 80 kDa. The expected band size for SCIN is at 80 kDa.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For SCIN (Source: Uniprot.org, NCBI)
Gene Name
SCIN
Full Name
Adseverin
Weight
80 kDa
Superfamily
villin/gelsolin family
Alternative Names
Trophoblast glycoprotein;5T4 oncofetal antigen;5T4 oncofetal trophoblast glycoprotein;5T4 oncotrophoblast glycoprotein;M6P1;Wnt-activated inhibitory factor 1;WAIF1;TPBG;5T4; Scin|AW545522, ads, adseverin|scinderin|adseverin|gelsolin-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCIN, check out the SCIN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCIN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SCIN Rabbit Monoclonal Antibody (M07463)
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