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Product Info Summary
| SKU: | EK1933 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) and urine. |
Product info
Product Name
Human CFHR5 ELISA Kit PicoKine®
View all Complement Factor H-related 5/CFHR5 ELISA kits
SKU/Catalog Number
EK1933
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CFHR5 ELISA Kit PicoKine® (96 Tests). Quantitate Human CFHR5 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CFHR5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1933)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E19-E569
Sensitivity
<75 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1933 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1933 is reactive to CFHR5 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA) and urine.
Application Guarantee
EK1933 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Complement Factor H-related 5/CFHR5
Complement factor H-related protein 5 is a protein that in humans is encoded by the CFHR5 gene. It is mapped to 1q31.3. This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS).
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1933-CAP | Anti-Human CFHR5 Pre-coated 96-well strip microplate | 1 |
| EK1933-ST | Human CFHR5 Standard | 2 vials, 50 ng/tube |
| EK1933-DA | Human CFHR5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.024 | 0.121 | 0.163 | 0.289 | 0.622 | 0.906 | 1.573 | 2.081 |
Data Example Images
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Human CFHR5 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Complement Factor H-related 5/CFHR5, Dilution ratio of 1:1000, concentration in serum and plasma is 1.6-3.1 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CFHR5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 190 | 432 | 2544 | 294 | 510 | 4693 |
| Standard deviation | 12.24 | 18.14 | 170.19 | 12.94 | 36.77 | 334.61 |
| CV (%) | 6.4% | 4.2% | 6.7% | 4.4% | 7.2% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Complement Factor H-related 5/CFHR5 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 230 | 223 | 199 | 303 | 239 | 8.69 | 3.6% |
| Sample 2 | 321 | 623 | 589 | 427 | 490 | 38.51 | 7.9% |
| Sample 3 | 2847 | 4318 | 3088 | 2545 | 3200 | 167.97 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For CFHR5 (Source: Uniprot.Org, NCBI)
Gene Name
CFHR5
Full Name
Complement factor H-related protein 5
Weight
64.419kDa
Alternative Names
Complement factor H-related protein 5; FHR-5; CFHR5; CFHL5; FHR5 CFHR5 CFHL5D, FHR-5, FHR5, CFHR5 complement factor H related 5 complement factor H-related protein 5|factor H-related protein 5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CFHR5, check out the CFHR5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CFHR5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CFHR5 ELISA Kit PicoKine® (EK1933)
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human CFHR5 ELISA Kit PicoKine®
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CFHR5 in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
M. Dhar
Verified customer
Asked: 2019-10-04
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CFHR5 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-10-04
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-08-17
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-08-17
Question
Q: which procedure should I follow in order to thaw whole blood sample for CFHR5 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CFHR5 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-07-01
Question
Q: What is the optimal O.D. value for CFHR5 ELISA kit? I used your CFHR5 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CFHR5 even though the O.D. values are not very high?
B. Dhawan
Verified customer
Asked: 2019-01-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CFHR5 ELISA kit to have sensitivity of 0.1ng/ml, that means the minimum amount of CFHR5 that can be declared/interpreted as positive by the above standard is 0.1ng/ml.
Boster Scientific Support
Answered: 2019-01-02
Question
Q: if the enzyme conjugated CFHR5 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CFHR5 antigen?
Verified Customer
Verified customer
Asked: 2018-09-16
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-09-16
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-09-04
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2017-11-07
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-11-07
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
W. Wright
Verified customer
Asked: 2017-06-29
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-06-29
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CFHR5 using the EK1933 Human CFHR5 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-02-28
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2017-02-28
