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Product Info Summary
| SKU: | A08117 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-JMJD1B KDM3B Antibody
SKU/Catalog Number
A08117
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-JMJD1B KDM3B Antibody (Catalog # A08117). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
JMJD1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-JMJD1B KDM3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08117)
Host
Rabbit
Contents
JMJD1B Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
JMJD1B antibody was raised against a 20 amino acid synthetic peptide from near the center of human JMJD1B. The immunogen is located within amino acids 1050 - 1100 of JMJD1B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody will not cross-react with JMJD1A or JMJD1C.
Reactive Species
A08117 is reactive to KDM3B in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
191581 MW
Background of JMJD1B
The jumonji domain containing 1B protein (JMJD1B) was originally discovered as a candidate for the myeloid leukemia tumor suppressor gene. Besides CD34+ cells and acute myeloid leukemia (AML) cell lines, JMJD1B mRNA is highly expressed in liver, heart, kidney, skeletal muscle, and placenta tissues. The JMJD1B gene is localized to a region of chromosome 5q31, which is frequently deleted in myeloid leukemias and myelodysplasias and expression of JMJD1B in a del(5q) cell line results in suppression of clonogenic growth suggesting that JMJD1B may function as a tumor suppressor. In contrast, JMJD1B gene copy number and mRNA expression level was increased in several non-small cell lung cancers indicating that the role of JMJD1B in cancer formation and progression is more complex than originally postulated. At least three isoforms of JMJD1B are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08117 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
JMJD1B antibody can be used for detection of JMJD1B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of JMJD1B in rat liver tissue lysate with JMJD1B antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of JMJD1B in rat liver tissue with JMJD1B antibody at 2.5 μg/mL.
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Immunofluorescence of JMJD1B in rat liver tissue with JMJD1B antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For KDM3B (Source: Uniprot.org, NCBI)
Gene Name
KDM3B
Full Name
Lysine-specific demethylase 3B
Weight
191581 MW
Superfamily
JHDM2 histone demethylase family
Alternative Names
5qNCA, NET22, C5orf7, JMJD1B, JHDM2B, KIAA1082, Lysine-specific demethylase 3B, JmjC domain-containing histone demethylation protein 2B KDM3B 5qNCA, C5orf7, DIJOS, JMJD1B, NET22 lysine demethylase 3B lysine-specific demethylase 3B|[histone H3]-dimethyl-L-lysine(9) demethylase 3B|jmjC domain-containing histone demethylation protein 2B|jumonji domain containing 1B|jumonji domain-containing protein 1B|lysine (K)-specific demethylase 3B|nuclear protein 5qNCA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KDM3B, check out the KDM3B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KDM3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JMJD1B KDM3B Antibody (A08117)
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