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Product Info Summary
| SKU: | EK1399 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Human Complement Factor H/CFH ELISA Kit PicoKine®
View all Complement Factor H ELISA kits
SKU/Catalog Number
EK1399
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Complement Factor H/CFH ELISA Kit PicoKine® (96 Tests). Quantitate Human CFH in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Complement Factor H/CFH ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1399)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Target protein isolated from human plasma
Sensitivity
<50 pg/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
See datasheet of EK1399 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1399 is reactive to CFH in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK1399 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Complement Factor H
Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1399-CAP | Anti-Human CFH Pre-coated 96-well strip microplate | 1 |
| EK1399-ST | Human CFH Standard | 2 vials, 100 ng/tube |
| EK1399-DA | Human CFH Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.017 | 0.135 | 0.233 | 0.445 | 0.857 | 1.343 | 1.813 | 2.017 |
Data Example Images
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Human Complement H/CFH PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Complement Factor H, Dilution ratio of 1:20000, concentration in serum and plasma is around 300 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Complement Factor H/CFH ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 3203 | 13518 | 53844 | 3406 | 12935 | 57149 |
| Standard deviation | 233.81 | 784.04 | 3822.92 | 303.13 | 905.45 | 4971.96 |
| CV (%) | 7.3% | 5.8% | 7.1% | 8.9% | 7% | 8.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Complement Factor H in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 3203 | 2994 | 3170 | 3050 | 3104 | 85.4 | 2.7% |
| Sample 2 | 13518 | 12944 | 13467 | 11714 | 12910 | 726.54 | 5.6% |
| Sample 3 | 53844 | 53833 | 53094 | 52680 | 53362 | 497.77 | 0.9% |
Protein Target Info & Infographic
Gene/Protein Information For CFH (Source: Uniprot.Org, NCBI)
Gene Name
CFH
Full Name
Complement factor H
Weight
139096 MW
Alternative Names
Complement factor H;H factor 1;CFH;HF, HF1, HF2; CFH AHUS1, AMBP1, ARMD4, ARMS1L3, FH, FHL1, HF, HF1, HF2, HUS, CFH complement factor H complement factor H|H factor 1 (complement)|H factor 2 (complement)|adrenomedullin binding protein|age-related maculopathy susceptibility 1|beta-1-H-globulin|beta-1H|factor H|factor H-like 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CFH, check out the CFH Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CFH: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Complement Factor H/CFH ELISA Kit PicoKine® (EK1399)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human Complement Factor H/CFH ELISA Kit PicoKine®
Question
Q: is it okay to use citrate plasma as samples in Human Complement Factor H Picokine® ELISA Kit (Catalog # EK1399)?
K. Zhang
Verified customer
Asked: 2020-10-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Complement Factor H causing disruption of its protein structure. Complement Factor H may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Complement Factor H ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-10-26
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-08-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-08-30
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
W. Miller
Verified customer
Asked: 2020-06-12
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-06-12
Question
Q: can you recommend the dilution ratio of serum samples for detection of Complement Factor H in Human urine? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
S. Chakrabarti
Verified customer
Asked: 2020-03-07
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Complement Factor H ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-03-07
Question
Q: how to proceed with the analysis of ELISA data? I have obtained Complement Factor H level in urine.
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-09-06
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-08-16
Question
Q: if the enzyme conjugated Complement Factor H antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Complement Factor H antigen?
Y. Li
Verified customer
Asked: 2018-06-12
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-06-12
