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Product Info Summary
| SKU: | PB9502 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband®
SKU/Catalog Number
PB9502
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband® catalog # PB9502. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9502)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse SCARB1, different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9502 is reactive to Scarb1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
85 kDa
Calculated molecular weight
56754 MW
Background of SCARB1
Scavenger receptor class B member 1 (SRB1), also known as SR-BI, is a protein that in humans is encoded by the SCARB1 gene. SR-BI functions as a receptor for high-density lipoprotein. Scavenger receptor class B, type I (SR-BI) is an integral membrane protein found in numerous cell types/tissues, including the liver and adrenal. It is best known for its role in facilitating the uptake of cholesteryl esters from high-density lipoproteins in the liver. This process drives the movement of cholesterol from peripheral tissues towards the liver for excretion. This movement of cholesterol is known as reverse cholesterol transport and is a protective mechanism against the development of atherosclerosis, which is the principal cause of heart disease and stroke. SR-BI has also been identified in the livers of non-mammalian species (turtle, goldfish, shark, chicken, frog, and skate), suggesting it emerged early in vertebrate evolutionary history. The turtle also seems to upregulate SB-RI during egg development, indicating that cholesterol efflux may be at peak levels during developmental stages.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9502 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry, 2-5 μg/ml, Rat
Positive Control
WB: rat liver tissue, mouse liver tissue
IHC: rat adrenal gland tissue, rat adrenal gland tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SCARB1 using anti-SCARB1 antibody (PB9502).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCARB1 antigen affinity purified polyclonal antibody (Catalog # PB9502) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCARB1 at approximately 85 kDa. The expected band size for SCARB1 is at 57 kDa.
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Figure 2. IHC analysis of SCARB1 using anti-SCARB1 antibody (PB9502).
SCARB1 was detected in a paraffin-embedded section of rat adrenal gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCARB1 Antibody (PB9502) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of SCARB1 using anti-SCARB1 antibody (PB9502).
SCARB1 was detected in a paraffin-embedded section of rat adrenal gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCARB1 Antibody (PB9502) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Scarb1 (Source: Uniprot.org, NCBI)
Gene Name
Scarb1
Full Name
Scavenger receptor class B member 1
Weight
56754 MW
Superfamily
CD36 family
Alternative Names
Scavenger receptor class B member 1;SRB1;SR-BI;Scarb1;Srb1; SCARB1 CD36L1, CLA-1, CLA1, HDLQTL6, SR-BI, SRB1 scavenger receptor class B member 1 scavenger receptor class B member 1|CD36 and LIMPII analogous 1|CD36 (collagen type I receptor, thrombospondin receptor)-like 1|scavenger receptor class B type III
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Scarb1, check out the Scarb1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Scarb1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband® (PB9502)
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Customer Q&As
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1 Customer Q&As for Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband®
Question
We are currently using anti-Scavenging Receptor SR-BI/SCARB1 antibody PB9502 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
The anti-Scavenging Receptor SR-BI/SCARB1 antibody (PB9502) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-21
