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Key=meta_title, value=Scavenging Receptor SR-BI/SCARB1 Antibody
Key=meta_description, value=Boster Bio Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband® catalog # PB9502. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=image_label, value=Figure 1. Western blot analysis of SCARB1 using anti-SCARB1 antibody (PB9502).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sampl
Key=small_image_label, value=Figure 1. Western blot analysis of SCARB1 using anti-SCARB1 antibody (PB9502).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sampl
Key=thumbnail_label, value=Figure 1. Western blot analysis of SCARB1 using anti-SCARB1 antibody (PB9502).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sampl
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Key=size, value=100 μg/vial
Key=protein_name, value=Scavenger receptor class B member 1
Key=gene_full_name, value=Scavenger receptor class B member 1
Key=uniprot_id, value=Q61009
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=sensitivity
Key=applications, value=IHC, WB
Key=reactivity, value=Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Key=predicted_reactivity
Key=source_company, value=Boster PB9502
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=56754 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Cancer, Cancer Metabolism, Cardiovascular, Cholesterol Metabolism, Host-Virus Interaction, Interspecies Interaction, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Microbiology, Pathways and Processes, Signal Transduction
Key=clonality, value=Polyclonal
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Key=cj_related_products, value=Cholesterol Esterification:PA1967|PA1882^Cholesterol Metabolism:A02378-1^Intestinal Cholesterol Absorption:A01601|PA1007-1|A04840
Key=description, value=Boster Bio Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband® catalog # PB9502. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband® catalog # PB9502. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband®
Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Key=background, value=Scavenger receptor class B member 1 (SRB1), also known as SR-BI, is a protein that in humans is encoded by the SCARB1 gene. SR-BI functions as a receptor for high-density lipoprotein. Scavenger receptor class B, type I (SR-BI) is an integral membrane protein found in numerous cell types/tissues, including the liver and adrenal. It is best known for its role in facilitating the uptake of cholesteryl esters from high-density lipoproteins in the liver. This process drives the movement of cholesterol from peripheral tissues towards the liver for excretion. This movement of cholesterol is known as reverse cholesterol transport and is a protective mechanism against the development of atherosclerosis, which is the principal cause of heart disease and stroke. SR-BI has also been identified in the livers of non-mammalian species (turtle, goldfish, shark, chicken, frog, and skate), suggesting it emerged early in vertebrate evolutionary history. The turtle also seems to upregulate SB-RI during egg development, indicating that cholesterol efflux may be at peak levels during developmental stages.
Key=gene_name, value=Scarb1
Key=synonyms, value=Scavenger receptor class B member 1;SRB1;SR-BI;Scarb1;Srb1;
Key=application_details, value=Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry, 2-5 μg/ml, Rat

Key=subcellular_localization, value= Cell membrane ; Multi-pass membrane protein . Membrane, caveola ; Multi-pass membrane protein . Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae. .
Key=tissue_specificity, value= Expressed primarily in liver and non-placental steroidogenic tissues.
Key=protein_function, value= Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity (By similarity). Plays an important role in the uptake of HDL cholesteryl ester (By similarity). .
Key=application_notes, value=Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 32342|sc 32350|sc 67098|sc 67099
Key=immunogen, value=A synthetic peptide corresponding to a sequence at the C-terminus of mouse SCARB1, different from the related rat sequence by one amino acid.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=85 kDa
Key=custom_attribute_2, value=WB: rat liver tissue, mouse liver tissue
IHC: rat adrenal gland tissue, rat adrenal gland tissue
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Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband®

SCARB1 antibody

Boster Bio Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband® catalog # PB9502. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9502
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband®

View all SCARB1 Antibodies

SKU/Catalog Number

PB9502

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband® catalog # PB9502. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9502)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse SCARB1, different from the related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9502 is reactive to Scarb1 in Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

85 kDa

Calculated molecular weight

56754 MW

Background of SCARB1

Scavenger receptor class B member 1 (SRB1), also known as SR-BI, is a protein that in humans is encoded by the SCARB1 gene. SR-BI functions as a receptor for high-density lipoprotein. Scavenger receptor class B, type I (SR-BI) is an integral membrane protein found in numerous cell types/tissues, including the liver and adrenal. It is best known for its role in facilitating the uptake of cholesteryl esters from high-density lipoproteins in the liver. This process drives the movement of cholesterol from peripheral tissues towards the liver for excretion. This movement of cholesterol is known as reverse cholesterol transport and is a protective mechanism against the development of atherosclerosis, which is the principal cause of heart disease and stroke. SR-BI has also been identified in the livers of non-mammalian species (turtle, goldfish, shark, chicken, frog, and skate), suggesting it emerged early in vertebrate evolutionary history. The turtle also seems to upregulate SB-RI during egg development, indicating that cholesterol efflux may be at peak levels during developmental stages.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PB9502 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry, 2-5 μg/ml, Rat

Positive Control

WB: rat liver tissue, mouse liver tissue
IHC: rat adrenal gland tissue, rat adrenal gland tissue

Validation Images & Assay Conditions

Gene/Protein Information For Scarb1 (Source: Uniprot.org, NCBI)

Gene Name

Scarb1

Full Name

Scavenger receptor class B member 1

Weight

56754 MW

Superfamily

CD36 family

Alternative Names

Scavenger receptor class B member 1;SRB1;SR-BI;Scarb1;Srb1; SCARB1 CD36L1, CLA-1, CLA1, HDLQTL6, SR-BI, SRB1 scavenger receptor class B member 1 scavenger receptor class B member 1|CD36 and LIMPII analogous 1|CD36 (collagen type I receptor, thrombospondin receptor)-like 1|scavenger receptor class B type III

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Scarb1, check out the Scarb1 Infographic

Scarb1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Scarb1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband®

Question

We are currently using anti-Scavenging Receptor SR-BI/SCARB1 antibody PB9502 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2017-09-21

Answer

The anti-Scavenging Receptor SR-BI/SCARB1 antibody (PB9502) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-09-21

Order DetailsPrice
PB9502

100μg

$370
PB9502-10ug

10μg sample (liquid)

$99
PB9502-Biotin

100 μg Biotin conjugated

$570
PB9502-Cy3

100 μg Cy3 conjugated

$570
PB9502-Dylight488

100 μg Dylight488 conjugated

$570
PB9502-Dylight550

100 μg Dylight550 conjugated

$570
PB9502-Dylight594

100 μg Dylight594 conjugated

$570
PB9502-FITC

100 μg FITC conjugated

$570
PB9502-HRP

100 μg HRP conjugated

$570
PB9502-APC

100 μg APC conjugated

$670
PB9502-PE

100 μg PE conjugated

$670
PB9502-iFluor647

100 μg iFluor647 conjugated

$670
PB9502-carrier-free

Carrier Free

$370

More conjugates/formats

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PB9502
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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