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Product Info Summary
| SKU: | PA1967 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-LCAT Antibody Picoband®
SKU/Catalog Number
PA1967
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LCAT Antibody catalog # PA1967. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LCAT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1967)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human LCAT, different from the related rat sequence by two amino acids, and from the mouse sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1967 is reactive to LCAT in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
49578 MW
Background of LCAT
LCAT (Lecithin: Cholesterol Acyltransferase), is an enzyme that converts free cholesterol into cholesteryl ester. Azoulay et al. (1987) used a cDNA clone corresponding to LCAT to assign the locus to 16q22 through the analysis of DNA from somatic cell hybrids and in situ hybridization. LCAT plays an important role in lipoprotein metabolism, especially in the process termed "reverse cholesterol transport" The enzyme is synthesized in the liver and circulates in blood plasma as a complex with components of high density lipoprotein (HDL). Cholesterol from peripheral cells is transferred to HDL particles, esterified through the action of LCAT on HDL, and incorporated into the core of the lipoprotein. The cholesterol ester is thereby transported to the liver (Jonas, 2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1967 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Brain Tissue, U87 Cell, HELA Cell, SMMC Cell,
Validation Images & Assay Conditions
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Anti-LCAT antibody, PA1967, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: U87 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: SMMC Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For LCAT (Source: Uniprot.org, NCBI)
Gene Name
LCAT
Full Name
Phosphatidylcholine-sterol acyltransferase
Weight
49578 MW
Superfamily
AB hydrolase superfamily
Alternative Names
Phosphatidylcholine-sterol acyltransferase;2.3.1.43;Lecithin-cholesterol acyltransferase;Phospholipid-cholesterol acyltransferase;LCAT; LCAT lecithin-cholesterol acyltransferase phosphatidylcholine-sterol acyltransferase|1-alkyl-2-acetylglycerophosphocholine esterase|PAF acetylhydrolase|phosphatidylcholine--sterol O-acyltransferase|phospholipid-cholesterol acyltransferase|platelet-activating factor acetylhydrolase|testicular secretory protein Li 24
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LCAT, check out the LCAT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LCAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LCAT Antibody Picoband® (PA1967)
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No publications found for PA1967
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Customer Q&As
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7 Customer Q&As for Anti-LCAT Antibody Picoband®
Question
I was wanting to use your anti-LCAT antibody for WB for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
As indicated on the product datasheet, PA1967 anti-LCAT antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-13
Question
Will anti-LCAT antibody PA1967 work for WB with brain?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
According to the expression profile of brain, LCAT is highly expressed in brain. So, it is likely that anti-LCAT antibody PA1967 will work for WB with brain.
Boster Scientific Support
Answered: 2020-01-08
Question
My lab would like to test anti-LCAT antibody PA1967 on rat brain for research purposes, then I may be interested in using anti-LCAT antibody PA1967 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
The products we sell, including anti-LCAT antibody PA1967, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-26
Question
Do you have a BSA free version of anti-LCAT antibody PA1967 available?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-LCAT antibody PA1967 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-23
Question
Is a blocking peptide available for product anti-LCAT antibody (PA1967)?
Verified Customer
Verified customer
Asked: 2018-05-21
Answer
We do provide the blocking peptide for product anti-LCAT antibody (PA1967). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-21
Question
Would anti-LCAT antibody PA1967 work on zebrafish WB with plasma?
M. Brown
Verified customer
Asked: 2017-07-12
Answer
Our lab technicians have not tested anti-LCAT antibody PA1967 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-LCAT antibody PA1967 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish plasma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-12
Question
Will PA1967 anti-LCAT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Parker
Verified customer
Asked: 2015-07-28
Answer
It shows on the product datasheet, PA1967 anti-LCAT antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-07-28
