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Product Info Summary
| SKU: | PA1882 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Tyrosine-protein kinase Fer FER Antibody Picoband®
SKU/Catalog Number
PA1882
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tyrosine-protein kinase Fer FER Antibody catalog # PA1882. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tyrosine-protein kinase Fer FER Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1882)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human FER, different from the related rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1882 is reactive to FER in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
95 kDa
Calculated molecular weight
94638 MW
Background of FER
FER (FPS/FES-Related tyrosine kinase) also known as TYK3, is an enzyme that in humans is encoded by the FER gene. Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. By in situ hybridization, Morris et al. (1990) concluded that the FER gene is located at 5q21-q22. Treatment of cells with JMP resulted in the release of FER from the cadherin complex and its accumulation in the integrin complex. The accumulation of FER in the integrin complex and the inhibitory effects of JMP could be reversed with a peptide that mimics the first coiled-coil domain of FER. The results suggested that FER mediates crosstalk between CDH2 and ITGB1. In Fer mutant mice, leukocyte emigration was exaggerated in response to LPS without altering vascular permeability, suggesting that FER has a role in regulating innate immunity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1882 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: HELA Whole Cell, Rat Testis Tissue, Rat Ovary Tissue
IHC: Human Intestinal Cancer Tissue, Rat Intestine Tissue
Validation Images & Assay Conditions
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Anti-FER antibody, PA1882, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Anti-FER antibody, PA1882, IHC(P)
IHC(P): Rat Intestine Tissue
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Anti-FER antibody, PA1882, Western blotting
All lanes: Anti FER (PA1882) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: Rat Ovary Tissue Lysate at 50ug
Predicted bind size: 95KD
Observed bind size: 95KD
Protein Target Info & Infographic
Gene/Protein Information For FER (Source: Uniprot.org, NCBI)
Gene Name
FER
Full Name
Tyrosine-protein kinase Fer
Weight
94638 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase Fer;2.7.10.2;Feline encephalitis virus-related kinase FER;Fujinami poultry sarcoma/Feline sarcoma-related protein Fer;Proto-oncogene c-Fer;Tyrosine kinase 3;p94-Fer;FER;TYK3; FER PPP1R74, TYK3, p94-Fer FER tyrosine kinase tyrosine-protein kinase Fer|feline encephalitis virus-related kinase FER|fer (fps/fes related) tyrosine kinase|fujinami poultry sarcoma/Feline sarcoma-related protein Fer|phosphoprotein NCP94|protein phosphatase 1, regulatory subunit 74|proto-oncogene c-Fer|tyrosine kinase 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FER, check out the FER Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FER: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tyrosine-protein kinase Fer FER Antibody Picoband® (PA1882)
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Customer Q&As
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7 Customer Q&As for Anti-Tyrosine-protein kinase Fer FER Antibody Picoband®
Question
Is this PA1882 anti-FER antibody reactive to the isotypes of FER?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
The immunogen of PA1882 anti-FER antibody is A synthetic peptide corresponding to a sequence in the middle region of human FER(521-536aa FSNIPQLIDHHYTTKQ), different from the related rat and mouse sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-12
Question
I am looking for to test anti-FER antibody PA1882 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-FER antibody PA1882 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
The products we sell, including anti-FER antibody PA1882, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-05
Question
I see that the anti-FER antibody PA1882 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-04
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-04
Question
I was wanting to use your anti-FER antibody for IHC for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2018-04-20
Answer
You can see on the product datasheet, PA1882 anti-FER antibody has been validated for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-20
Question
Do you have a BSA free version of anti-FER antibody PA1882 available?
Verified Customer
Verified customer
Asked: 2017-11-30
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-FER antibody PA1882 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-30
Question
Does anti-FER antibody PA1882 work on primate WB with corpus callosum?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
Our lab technicians have not validated anti-FER antibody PA1882 on primate. You can run a BLAST between primate and the immunogen sequence of anti-FER antibody PA1882 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate corpus callosum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-07
Question
We are currently using anti-FER antibody PA1882 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on primate tissues as well?
A. Roberts
Verified customer
Asked: 2013-10-29
Answer
The anti-FER antibody (PA1882) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-10-29
