Product Info Summary
SKU: | M02588 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SATB2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02588
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SATB2 Rabbit Monoclonal Antibody catalog # M02588. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SATB2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02588)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADI-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SATB2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02588 is reactive to SATB2 in Human, Mouse, Rat
Applications
M02588 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
83 kDa
Calculated molecular weight
82555 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
![m02588 satb2 primary antibodies wb testing 1 m02588 satb2 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m02588-satb2-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of SATB2 using anti-SATB2 antibody (M02588).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SATB2 antigen affinity purified monoclonal antibody (Catalog # M02588) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SATB2 at approximately 83 kDa. The expected band size for SATB2 is at 83 kDa.
![m02588 wb7 m02588 wb7](https://www.bosterbio.com/media/catalog/product/m/0/m02588-wb7.jpg)
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
![M02588 SATB2 primary antibodies IHC testing 1 M02588 SATB2 primary antibodies IHC testing 1](https://www.bosterbio.com/media/catalog/product/M/0/M02588-SATB2-primary-antibodies-IHC-testing-1.jpg)
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Immunohistochemical analysis of paraffin-embedded human stomach cancer, using SATB2 Antibody.
![m02588 ihc7 m02588 ihc7](https://www.bosterbio.com/media/catalog/product/m/0/m02588-ihc7.jpg)
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Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:100 dilution.
![m02588 ihc8 m02588 ihc8](https://www.bosterbio.com/media/catalog/product/m/0/m02588-ihc8.jpg)
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Immunohistochemical analysis of paraffin-embedded Human cervical cancer, using the Antibody at 1:100 dilution.
![m02588 satb2 primary antibodies if testing 3 m02588 satb2 primary antibodies if testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m02588-satb2-primary-antibodies-if-testing-3.jpg)
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Immunofluorescent analysis of SH-SY5Y cells, using SATB2 Antibody .
![m02588 icc1 m02588 icc1](https://www.bosterbio.com/media/catalog/product/m/0/m02588-icc1.jpg)
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For SATB2 (Source: Uniprot.org, NCBI)
Gene Name
SATB2
Full Name
DNA-binding protein SATB2
Weight
82555 MW
Superfamily
CUT homeobox family
Alternative Names
DNA-binding protein SATB2; FLJ32076; KIAA1034FLJ21474; MGC119474; MGC119477; SATB family member 2; SATB homeobox 2; Special AT-rich sequence-binding protein 2 SATB2 GLSS SATB homeobox 2 DNA-binding protein SATB2|SATB family member 2|special AT-rich sequence-binding protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SATB2, check out the SATB2 Infographic
![SATB2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SATB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SATB2 Rabbit Monoclonal Antibody (M02588)
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