Anti-CLNS1A/Picln Antibody

Product Name

Anti-CLNS1A/Picln Antibody

View all CLNS1A Antibodies

SKU/Catalog Number

A06086

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-CLNS1A/Picln Antibody (Catalog # A06086). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CLNS1A/Picln Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06086)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from C-terminal of human CLNS1A.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06086 is reactive to CLNS1A in Human

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Observed Molecular Weight

Calculated molecular weight

26215 MW

Background of CLNS1A

Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also indirectly participate in cellular volume control by activation of a swelling-induced chloride conductance pathway.

Buyse G., Biochem. Biophys. Res. Commun. 218:822-827(1996).
Anquita J., Biochem. Biophys. Res. Commun. 208:89-95(1995).
Lamb F.S., Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06086 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Positive Control

Validation Images & Assay Conditions

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Western blot analysis of extracts from HuvEc cells, K562 cells, HeLa cells, HepG2 cells, A549 cells and Jurkat cells, using CLNS1A antibody A06086.

Gene/Protein Information For CLNS1A (Source: Uniprot.org, NCBI)

Alternative Names

chloride channel regulatory protein; Chloride channel, nucleotide sensitive 1A; chloride channel, nucleotide-sensitive, 1A; Chloride conductance regulatory protein ICln; Chloride ion current inducer protein; ClCI; CLNS1B; i(Cln); ICln; methylosome subunit pICln; Reticulocyte pICln; reticulocyte protein ICln CLNS1A CLCI, CLNS1B, ICln chloride nucleotide-sensitive channel 1A methylosome subunit pICln|chloride channel regulatory protein|chloride channel, nucleotide sensitive 1A|chloride conductance regulatory protein ICln|chloride ion current inducer protein|i(Cln)|reticulocyte pICln|reticulocyte protein ICln

For more info on CLNS1A, check out the CLNS1A Infographic

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In this infographic, you will see the following information for CLNS1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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