Anti-MAPKAP1 Antibody

Product Name

Anti-MAPKAP1 Antibody

View all Sin1/MAPKAP1 Antibodies

SKU/Catalog Number

A04068-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-MAPKAP1 Antibody (Catalog # A04068-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

MAPKAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-MAPKAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04068-1)

Host

Rabbit

Contents

MAPKAP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

MAPKAP1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human MAPKAP1. The immunogen is located within amino acids 90 - 140 of MAPKAP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

ADRM1 antibody is human, mouse and rat reactive. At least two isoforms of ADRM1 are know to exist; this antibody will detect both isoforms.

Reactive Species

A04068-1 is reactive to MAPKAP1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

59123 MW

Background of Sin1/MAPKAP1

MAPKAP1 was initially identified as the human homolog of S. pombe SIN1. Recent evidence has shown that it identical to Mip1, a protein that interacts with MEKK2, a member of the mitogen-activated protein kinase (MAPK) intracellular signaling network. MAPKAP1 is thought to prevent MEKK2 activation and dimerization by forming a complex with the inactive and non-phosphorylated MEKK2, thereby blocking the JNK1/2, ERK1/2, p38 and ERK5 MAPKs. MAPKAP1 has also been shown to play a role in the TOR signaling process, a pathway that is involved in controlling cell growth and proliferation in response to environmental cues such as nutrients, growth factors and hormones. Experiments showed that MAPKAP1 helped to maintain the TOR/rictor assembly but not the TOR/RAPTOR complex, which allowed specific phosphorylation of Akt, a kinase that is believed to couple the growth factor-PI3K signaling pathway to the nutrient-regulated TOR signaling pathway. Multiple alternatively spliced isoforms of MAPKAP1 have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04068-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MAPKAP1 antibody can be used for detection of MAPKAP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For MAPKAP1 (Source: Uniprot.org, NCBI)

Alternative Names

MIP1, SIN1, JC310, SIN1b, SIN1g, MIP1, Target of rapamycin complex 2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, TORC2 subunit MAPKAP1 MAPKAP1 JC310, MIP1, SIN1, SIN1b, SIN1g MAPK associated protein 1 target of rapamycin complex 2 subunit MAPKAP1|MEKK2-interacting protein 1|SAPK-interacting protein 1|TORC2 subunit MAPKAP1|mSIN1|mitogen-activated protein kinase 2-associated protein 1|mitogen-activated protein kinase associated protein 1|ras inhibitor MGC2745|stress-activated map kinase interacting protein 1|stress-activated protein kinase-interacting 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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