Anti-SAE2 / UBA2 Monoclonal Antibody

Product Name

Anti-SAE2 / UBA2 Monoclonal Antibody

View all SAE2 Antibodies

SKU/Catalog Number

M03816

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-SAE2 / UBA2 Monoclonal Antibody catalog # M03816. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SAE2 / UBA2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03816)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEAI-21

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human SAE2 / UBA2 The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03816 is reactive to UBA2 in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

43 kDa

Calculated molecular weight

48121 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03816 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Positive Control

WB: A549 cell

Validation Images & Assay Conditions

Gene/Protein Information For UBA2 (Source: Uniprot.org, NCBI)

Alternative Names

Protein disulfide-isomerase A6;5.3.4.1;Endoplasmic reticulum protein 5;ER protein 5;ERp5;Protein disulfide isomerase P5;Thioredoxin domain-containing protein 7;PDIA6;ERP5, P5, TXNDC7; UBA2 ARX, HRIHFB2115, SAE2 ubiquitin like modifier activating enzyme 2 SUMO-activating enzyme subunit 2|SUMO-1 activating enzyme subunit 2|SUMO1 activating enzyme subunit 2|UBA2, ubiquitin-activating enzyme E1 homolog|anthracycline-associated resistance ARX|ubiquitin-like 1-activating enzyme E1B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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