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Product Info Summary
| SKU: | PB9742 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MRP8/S100A8 Antibody Picoband®
SKU/Catalog Number
PB9742
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MRP8/S100A8 Antibody Picoband® catalog # PB9742. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP8/S100A8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9742)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse S100A8 recombinant protein (Position: P2-E89). Mouse S100A8 shares 58.6% and 80.5% amino acid (aa) sequence identity with human and rat S100A8, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with recombinant human S100A8 is observed.
Reactive Species
PB9742 is reactive to S100a8 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated molecular weight
10295 MW
Background of S100a8
S100 calcium-binding protein A8 (S100A8) is a protein that in humans is encoded by the S100A8 gene. It is also known as calgranulin A. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9742 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Mouse
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse
Positive Control
WB: rat spleen tissue, rat thymus tissue, mouse spleen tissue, mouse thymus tissue
IHC: rat spleen tissue, mouse spleen tissue
ICC/IF: NIH3T3 cell
FCM: HEPA 1-6 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of S100A8 using anti-S100A8 antibody (PB9742).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A8 antigen affinity purified polyclonal antibody (Catalog # PB9742) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A8 at approximately 11 kDa. The expected band size for S100A8 is at 11 kDa.
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Figure 2. IHC analysis of S100A8 using anti-S100A8 antibody (PB9742).
S100A8 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-S100A8 Antibody (PB9742) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of S100A8 using anti-S100A8 antibody (PB9742).
S100A8 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-S100A8 Antibody (PB9742) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of S100A8 using anti-S100A8 antibody (PB9742).
S100A8 was detected in immunocytochemical section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-S100A8 Antibody (PB9742) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 5. Flow Cytometry analysis of HEPA 1-6 cells using anti-S100A8 antibody (PB9742).
Overlay histogram showing HEPA 1-6 cells stained with PB9742 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A8 Antibody (PB9742, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For S100a8 (Source: Uniprot.org, NCBI)
Gene Name
S100a8
Full Name
Protein S100-A8
Weight
10295 MW
Superfamily
S-100 family
Alternative Names
Protein S100-A8;Calgranulin-A;Chemotactic cytokine CP-10;Leukocyte L1 complex light chain;Migration inhibitory factor-related protein 8;MRP-8;p8;Pro-inflammatory S100 cytokine;S100 calcium-binding protein A8;S100a8;Caga, Mrp8; S100A8 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8 S100 calcium binding protein A8 protein S100-A8|calgranulin A|calprotectin L1L subunit|cystic fibrosis |leukocyte L1 complex light chain|migration inhibitory factor-related protein 8|urinary stone protein band A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on S100a8, check out the S100a8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for S100a8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRP8/S100A8 Antibody Picoband® (PB9742)
Hello CJ!
PB9742 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Pretreatment tumor-related leukocytosis misleads positron emission tomography-computed tomography during lymph node staging in gynecological malignancies
Species: Human
Mabuchi S,Komura N,Sasano T,Shimura K,Yokoi E,Kozasa K,Kuroda H,Takahashi R,Kawano M,Matsumoto Y,Kato H,Hatazawa J,Kimura T.Pretreatment tumor-related leukocytosis misleads positron emission tomography-computed tomography during lymph node staging in gyne
Species: Human,Mouse,Rat
PB9742 usage in article: APP:IHC, SAMPLE:CERVICAL TISSUE, DILUTION:NA
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Customer Q&As
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18 Customer Q&As for Anti-MRP8/S100A8 Antibody Picoband®
Question
We are currently using anti-MRP8/S100A8 antibody PB9742 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
P. Parker
Verified customer
Asked: 2020-04-06
Answer
The anti-MRP8/S100A8 antibody (PB9742) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-06
Question
My team were content with the WB result of your anti-MRP8/S100A8 antibody. However we have seen positive staining in keratinocyte secreted. cytoplasm. cytoplasm, using this antibody. Is that expected? Could you tell me where is S100A8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
From literature, keratinocyte does express S100A8. Generally S100A8 expresses in secreted. cytoplasm. cytoplasm,. Regarding which tissues have S100A8 expression, here are a few articles citing expression in various tissues:
Ascites, Pubmed ID: 7695842
Keratinocyte, Pubmed ID: 1286667
Liver, Pubmed ID: 24275569
Neutrophil, Pubmed ID: 1326551
Skeletal muscle, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-03-25
Question
I was wanting to use to test anti-MRP8/S100A8 antibody PB9742 on mouse tongue for research purposes, then I may be interested in using anti-MRP8/S100A8 antibody PB9742 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
The products we sell, including anti-MRP8/S100A8 antibody PB9742, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-20
Question
We want using your anti-MRP8/S100A8 antibody for positive regulation of inflammatory response studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
We appreciate your inquiry. This PB9742 anti-MRP8/S100A8 antibody is validated on mouse spleen tissue, tissue lysate, rat spleen tissue. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-06
Question
Is this PB9742 anti-MRP8/S100A8 antibody reactive to the isotypes of S100A8?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The immunogen of PB9742 anti-MRP8/S100A8 antibody is E.coli-derived mouse S100A8 recombinant protein (Position: P2-E89). Mouse S100A8 shares 58.6% and 80.5% amino acid (aa) sequence identity with human and rat S100A8, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-10
Question
Does anti-MRP8/S100A8 antibody PB9742 work on dog IHC with liver?
T. Moore
Verified customer
Asked: 2019-11-22
Answer
Our lab technicians have not tested anti-MRP8/S100A8 antibody PB9742 on dog. You can run a BLAST between dog and the immunogen sequence of anti-MRP8/S100A8 antibody PB9742 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-22
Question
Would anti-MRP8/S100A8 antibody PB9742 work for IHC with tongue?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
According to the expression profile of tongue, S100A8 is highly expressed in tongue. So, it is likely that anti-MRP8/S100A8 antibody PB9742 will work for IHC with tongue.
Boster Scientific Support
Answered: 2019-10-24
Question
Can you help my question with product PB9742, anti-MRP8/S100A8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9742 anti-MRP8/S100A8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-03
Question
Do you have a BSA free version of anti-MRP8/S100A8 antibody PB9742 available?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MRP8/S100A8 antibody PB9742 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-23
Question
Is a blocking peptide available for product anti-MRP8/S100A8 antibody (PB9742)?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
We do provide the blocking peptide for product anti-MRP8/S100A8 antibody (PB9742). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-05
Question
We have tried in the past anti-MRP8/S100A8 antibody for IHC on liver in the past. I am using mouse, and I plan to use the antibody for WB next. My question regards examining liver as well as skeletal muscle in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
T. Dhar
Verified customer
Asked: 2019-06-19
Answer
I looked at the website and datasheets of our anti-MRP8/S100A8 antibody and it appears that PB9742 has been validated on mouse in both IHC and WB. Thus PB9742 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-19
Question
See below the WB image, lot number and protocol we used for tongue using anti-MRP8/S100A8 antibody PB9742. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-10
Question
Would PB9742 anti-MRP8/S100A8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
It shows on the product datasheet, PB9742 anti-MRP8/S100A8 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-27
Question
We have been able to see staining in rat neutrophil. Any tips? Is anti-MRP8/S100A8 antibody supposed to stain neutrophil positively?
J. Johnson
Verified customer
Asked: 2019-03-14
Answer
Based on literature neutrophil does express S100A8. Based on Uniprot.org, S100A8 is expressed in pharyngeal mucosa, tongue, skeletal muscle, neutrophil, ascites, keratinocyte, liver, among other tissues. Regarding which tissues have S100A8 expression, here are a few articles citing expression in various tissues:
Ascites, Pubmed ID: 7695842
Keratinocyte, Pubmed ID: 1286667
Liver, Pubmed ID: 24275569
Neutrophil, Pubmed ID: 1326551
Skeletal muscle, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-03-14
Question
I see that the anti-MRP8/S100A8 antibody PB9742 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-19
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-19
Question
Does PB9742 react with human samples?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
PB9742 does not react with human samples.
Boster Scientific Support
Answered: 2017-08-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tongue using anti-MRP8/S100A8 antibody PB9742. Let me know if you need anything else.
R. Johnson
Verified customer
Asked: 2016-02-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-02-08
Question
I was wanting to use your anti-MRP8/S100A8 antibody for IHC for mouse tongue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse tongue identification?
D. Bhatt
Verified customer
Asked: 2014-07-25
Answer
You can see on the product datasheet, PB9742 anti-MRP8/S100A8 antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse tongue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-25
