Anti-RTP801 DDIT4 Antibody

Product Name

Anti-RTP801 DDIT4 Antibody

View all DDIT4 Antibodies

SKU/Catalog Number

A02019

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-RTP801 DDIT4 Antibody (Catalog # A02019). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

RTP801 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-RTP801 DDIT4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02019)

Host

Rabbit

Contents

RTP801 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RTP801 antibody was raised against a 12 amino acid synthetic peptide from near the center of human RTP801. The immunogen is located within amino acids 90 - 140 of RTP801.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

SRPK1 antibody is human, mouse and rat reactive. Multiple isoforms of SRPK1 are known to exist; this antibody will detect all except isoforms b and e. SRPK1 antibody is predicted to not cross-react with SRPK2.

Reactive Species

A02019 is reactive to DDIT4 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

25371 MW

Background of DDIT4

RTP801 was initially identified as a gene induced by DNA damage, and later found to also be regulated by other cellular stresses such as hypoxia and glucocorticoid treatment. Recently, RTP801 has been shown to act as a mediator of tuberous sclerosis complex (TSC)-dependent regulation of the mammalian Target of Rapamycin (mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle. In response to energy stress, RTP801 inhibits mTOR function, resulting in dephosphorylation of downstream targets such as ribosomal protein S6 kinase 1 and 4EBP1 and decreasing cell growth. Disregulation of RTP801 may thus contribute to human tumorigenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02019 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RTP801 antibody can be used for the detection of RTP801 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For DDIT4 (Source: Uniprot.org, NCBI)

Alternative Names

Dig2, REDD1, REDD-1, RTP801, DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801 DDIT4 Dig2, REDD-1, REDD1 DNA damage inducible transcript 4 DNA damage-inducible transcript 4 protein|HIF-1 responsive protein RTP801|protein regulated in development and DNA damage response 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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