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Product Info Summary
| SKU: | P02019 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-REDD1 (Thr23/25) Antibody
SKU/Catalog Number
P02019
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-REDD1 (Thr23/25) Antibody (Catalog # P02019). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-REDD1 (Thr23/25) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02019)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr23/25 of human REDD1, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P02019 is reactive to DDIT4 in Human
Applications
P02019 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
25371 MW
Background of DDIT4
REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli (Schwarzer et al, 2005). REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress (Katiyar et al, 2009). It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A–DDB1–ROC1–b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr-25, Thr-23 and Ser-19 through the activity of GSK3b (Katiyar et al, 2009).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500
Validation Images & Assay Conditions
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Western blot of jurkat cell lysate showing specific immunolabeling of the ~34 kDa REDD1 protein phosphorylated at Thr23/25 in the first lane (-). Phosphospecificity is shown in the second lane (+) where Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).
Protein Target Info & Infographic
Gene/Protein Information For DDIT4 (Source: Uniprot.org, NCBI)
Gene Name
DDIT4
Full Name
DNA damage-inducible transcript 4 protein
Weight
25371 MW
Superfamily
DDIT4 family
Alternative Names
Dig2; DNA-damage-inducible transcript 4; FLJ20500; HIF-1 responsive RTP801 (RTP801); Protein regulated in development and DNA damage response 1; REDD-1DNA damage-inducible transcript 4 protein; REDD1HIF-1 responsive protein RTP801; RTP801RP11-442H21.1 DDIT4 Dig2, REDD-1, REDD1 DNA damage inducible transcript 4 DNA damage-inducible transcript 4 protein|HIF-1 responsive protein RTP801|protein regulated in development and DNA damage response 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDIT4, check out the DDIT4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDIT4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-REDD1 (Thr23/25) Antibody (P02019)
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No publications found for P02019
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Phospho-REDD1 (Thr23/25) Antibody
Question
I am interested in to test anti-Phospho-REDD1 (Thr23/25) antibody P02019 on human fetal brain for research purposes, then I may be interested in using anti-Phospho-REDD1 (Thr23/25) antibody P02019 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
The products we sell, including anti-Phospho-REDD1 (Thr23/25) antibody P02019, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-10
Question
We are currently using anti-Phospho-REDD1 (Thr23/25) antibody P02019 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
The anti-Phospho-REDD1 (Thr23/25) antibody (P02019) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-23
Question
Will P02019 anti-Phospho-REDD1 (Thr23/25) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Jha
Verified customer
Asked: 2019-04-08
Answer
As indicated on the product datasheet, P02019 anti-Phospho-REDD1 (Thr23/25) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-Phospho-REDD1 (Thr23/25) antibody P02019. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-27
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-27
Question
Would anti-Phospho-REDD1 (Thr23/25) antibody P02019 work on canine WB with fetal brain?
Verified Customer
Verified customer
Asked: 2017-07-11
Answer
Our lab technicians have not tested anti-Phospho-REDD1 (Thr23/25) antibody P02019 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Phospho-REDD1 (Thr23/25) antibody P02019 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine fetal brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-11
Question
My question regarding product P02019, anti-Phospho-REDD1 (Thr23/25) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Banerjee
Verified customer
Asked: 2015-06-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P02019 anti-Phospho-REDD1 (Thr23/25) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-06-24
Question
Is this P02019 anti-Phospho-REDD1 (Thr23/25) antibody reactive to the isotypes of DDIT4?
T. Krishna
Verified customer
Asked: 2013-09-24
Answer
The immunogen of P02019 anti-Phospho-REDD1 (Thr23/25) antibody is The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr23/25 of human REDD1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-09-24
