RTCA Antibodies

RNA 3'-terminal phosphate cyclase (RTCA)

Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of this enzyme occurs in 3 steps: (A) adenylation of this enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A; (C) and assault of the adjacent 2'-hydroxyl on the 3'- phosphorus at the diester linkage to generate the cyclic end product.

The biological role of this enzyme is unknown but it is very likely to function in some aspects of cellular RNA processing. .

Gene Information

Human
Gene Name:	RTCA
Uniprot:	O00442
Entrez:	8634

Family

Belongs to:
RNA 3'-terminal cyclase family

Alternative Names

phosphate cyclase; RNA 3'-terminal phosphate cyclase; RNA cyclase; RNA terminal phosphate cyclase domain 1; RNA terminal phosphate cyclase domain-containing protein 1; RNA-3'-phosphate cyclase; RPC1; RPCEC 6.5.1.4; RTC domain containing 1; RTC domain-containing protein 1; RTC1

Molecular Weight

Mass (kDA):
39.337 kDA

Genomic Context

Human
Location:	1p21.2
Sequence:	1; NC_000001.11 (100266158..100292769)

Tissue Specificity

Ubiquitous.

Subcellular Localizations

Nucleus, nucleoplasm.

Diseases

• Malignant Neoplasms
• Malignant Neoplasm Of Lung
• Influenza
• Cardiac Arrhythmia
• Influenza Due To Influenza A Virus Subtype H1n1
• Malignant Neoplasm Of Breast
• Exudative Age-related Macular Degeneration
• Age Related Macular Degeneration
• Tumor Angiogenesis
• Neoplasms
• Pathologic Neovascularization
• Cell Invasion

• Cytopathogenic Effect, Viral
• Non-small Cell Lung Carcinoma
• Encephalitis, St. Louis
• Palmar-plantar Erythrodysesthesia Syndrome
• Malignant Tumor Of Cervix
• Torsades De Pointes
• Carcinoma

Pathways

• Cell Growth
• Cell Proliferation
• Reductive Tricarboxylic Acid Cycle
• Cytolysis
• Cell Migration
• Tricarboxylic Acid Cycle
• Induction Of Apoptosis
• Tube Formation
• Angiogenesis
• Rna Repair
• Immune Response

• Proteolysis
• Localization
• Light Absorption
• Notch Signaling Pathway
• Dna Replication
• Pathogenesis

PTMs

• Cleavage
• Phosphorylation
• Carboxylation

• Reduction
• Decarboxylation
• Oxidation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RTCA

Boster Bio - Best Uses Of The RTCA Marker

Boster offers high affinity antibodies that can be used in a variety of applications if you need primary antibodies. These antibodies have been consistently referenced and vetted by the research community over the past 25 years. Their antibodies are validated on Western Blotting, Immunohistochemistry, and ELISA. This article will give you an overview of all the Boster products that can be used for RTCA analysis.

Boster's high affinity primary antibodies

Boster Bio supplies high-affinity ELISA kits and antibodies. These products are rigorously tested and are available in a variety of formats, including WB or ELISA. In addition to primary antibodies, Boster also manufactures a variety of reagents and kits for various applications, including immunohistochemistry, cell culture, and neuroscience. The company's Quality Guarantee assures that each product will work as promised.

High-affinity primaries antibodies are highly useful tools for detecting proteins that have a wide range molecularweights. They have a high affinity for target protein and are sensitive to RTCA marks. In contrast to traditional monoclonal antibodies, high-affinity primary antibodies are made from a mixture of different species. The murine StrepMAB–Classic antibody, for example, is a good choice when staining proteins that have been tagged with the Strep protein group.

Another reason to choose a high affinity antibody is that it can be used in multiple detection methods. Depending upon your research needs, you may use multiple primary antibodies simultaneously or alternately between them. If you have more than one primary antibody, you can use them simultaneously, but the risk of cross-reactivity is high. When choosing a primary antibody to complement your research, you should take into account the specificity of these antibodies.

It is important that you choose the right primary antigen. The RTCA marker, however, is particularly useful for mIHC purposes. Multiple antibodies will allow you to ask more questions about your specimen. Combining a secondary antibody with your primary antibody will provide more robust results and more context data. For example, you can test whether a specific protein is associated with a certain RTCA marker or not, which can help you better understand the nature of the infection.

Tissue or cell Total Protein Extraction Kit

The BCA protein assay kits are designed to quantify isolated proteins. This kit uses RTCA to calculate the protein concentrations. It has been optimized to produce results within 90 minutes and minimal cross contamination of membrane fractions. Optimized reagents are used to extract the protein fast and thoroughly. For downstream applications, BCA protein assay kits are ideal.

This comprehensive kit contains all necessary reagents for the extraction of nuclear and cellular proteins. The kit includes a protease inhibitor cocktail to inhibit degradation of proteins. This results in a high-quality, high-quality protein, suitable for downstream uses such as SDS/PAGE.

Boster Bio Tissue, or Cell Total Protein Extractor, is a versatile tool that produces high-quality results. The RTCA marker detects RTCA in samples. This allows you to compare samples and determine quality. The kit includes all the necessary components, along with detailed troubleshooting. These kits are extremely efficient, and there is little risk of cross contamination.

BCA Protein Quantitative Kit

BosterBio RTCA Protein quantitation kit is what you want. The company offers detailed product information as well as BeNeLux delivery. Contact them to discuss custom orders. This kit will help you determine the protein content of different types of samples. For more information, visit the Boster Bio website. Sanbio can be reached for more information on Boster Bio.

The antibodies of the company are very specific and have a high affinity for protein samples. Boster's antibodies are backed by extensive, published research in prestigious journals. They are extremely sensitive and specific. They have been validated for use in Western Blotting, Immunohistochemistry, and ELISA. You can also get the protocol and images upon request.

iCell(r), Cardiomyocytes

iCell(r), Cardiomycells, are purified human hearts cells derived via hiPSCs. These cells are available in cryopreserved singlecell suspensions that include a mixture myocytes and electrically active meocytes. They have the same biochemical and electrophysiological characteristics as normal human heart cells, and they respond to exogenous agents in the expected manner.

Fujifilm Cellular Dynamics International uses a standard protocol to generate iCell® cardiomyocytes. These cell lines were selected to reflect the heterogeneity within the study population. The assay was subject to several biochemical testing to assess intra-individual variability.

In addition to analyzing the composition of the RTCA marker, iCell(r) Cardiomycells can also identify a subpopulation of cardiomyocytes with high pro-regenerative potential. These cells were first isolated from infants suffering from dilated heart disease. They were absent in children six years and older. While the adult heart has limited regenerative capacity, there are numerous uses for the RTCA marker.

In addition to cardiomyocytes, fibroblasts may be involved in immune responses. They are not as well studied as immune cells in a heart. They have been shown to contribute to the formation of extracellular matrix. They are also involved with cardiac rhythmicity. They comprise between 25-32% and 32% of the heart cells, respectively, in the absence or disease.

iCell(r), cardiomycells are an effective marker for identifying atrial or ventricular cardiomyocytes. These cells play a major role in the cardiac conduction system, transducing electrical stimuli to cause cardiac contraction. They are typically identified by the expression of a hyperpolarization-activated potassium channel (HPAC) and contactin-2, among other markers. These cells are best used with a single-nuclei-RNA-seq system and a mouse cell type Atlas.

To evaluate their cardiotoxicity, iCell(r), Cardiacmycells were exposed high doses ETP. The morphological changes seen in the iCell-CMs included vacuolization and sarcoplasmic sarcoplasm. The RTCA marker can also be used to detect changes in the structure and size of cellular organs.