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Product Info Summary
| SKU: | EK1641 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. |
Product info
Product Name
Human Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine®
View all Vitamin D BP ELISA kits
SKU/Catalog Number
EK1641
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Vitamin DBP ELISA Kit PicoKine® (96 Tests). Quantitate Human GC in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 150pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1641)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L17-L474
Sensitivity
<150 pg/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
See datasheet of EK1641 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1641 is reactive to GC in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
Application Guarantee
EK1641 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Vitamin D BP
Vitamin D-binding protein, also/originally known as gc-globulin (group-specific component), is a protein that in humans is encoded by the GC gene.The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1641-CAP | Anti-Human GC Pre-coated 96-well strip microplate | 1 |
| EK1641-ST | Human GC Standard | 2 vials, 100 ng/tube |
| EK1641-DA | Human GC Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.008 | 0.103 | 0.175 | 0.316 | 0.584 | 0.992 | 1.600 | 2.167 |
Data Example Images
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Human Vitamin DBP PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Vitamin D BP, Dilution ratio of 1:1, concentration in serum and plasma is 0-98 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 3726 | 12058 | 49927 | 3646 | 11478 | 49590 |
| Standard deviation | 182.57 | 940.52 | 2346.56 | 236.99 | 1033.02 | 72578.68 |
| CV (%) | 4.9% | 7.8% | 4.7% | 6.5% | 9% | 5.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Vitamin D BP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 3726 | 3810 | 3849 | 3865 | 3812 | 53.79 | 1.4% |
| Sample 2 | 12058 | 12135 | 11622 | 10357 | 11543 | 712.13 | 6.1% |
| Sample 3 | 49927 | 48196 | 52754 | 50855 | 50433 | 1645.09 | 3.2% |
Protein Target Info & Infographic
Gene/Protein Information For GC (Source: Uniprot.Org, NCBI)
Gene Name
GC
Full Name
Vitamin D-binding protein
Weight
52.918kDa
Superfamily
ALB/AFP/VDB family
Alternative Names
Vitamin D-binding protein; DBP; VDB; Gc protein-derived macrophage activating factor GC DBP, DBP-maf, DBP/GC, GRD3, Gc-MAF, GcMAF, HEL-S-51, VDB, VDBG, VDBP GC vitamin D binding protein vitamin D-binding protein|epididymis secretory protein Li 51|gc protein-derived macrophage activating factor|gc-globulin|group-specific component (vitamin D binding protein)|vitamin D-binding alpha-globulin|vitamin D-binding protein-macrophage activating factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GC, check out the GC Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GC: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine® (EK1641)
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No publications found for EK1641
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated Vitamin D BP antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Vitamin D BP antigen?
Verified Customer
Verified customer
Asked: 2020-09-07
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-09-07
Question
Q: can I use citrate plasma as samples in Human Vitamin D BP Picokine® ELISA Kit (Catalog # EK1641)?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Vitamin D BP causing degradation of its protein structure. Vitamin D BP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Vitamin D BP ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-03-11
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-01-04
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-01-04
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Vitamin D BP using the EK1641 Human Vitamin D BP Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-09-24
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-04-30
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-04-30
Question
Q: Can Vitamin D BP ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-09-26
Question
Q: how to analyze ELISA data? I have performed Vitamin D BP level in human milk.
B. King
Verified customer
Asked: 2018-06-26
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-06-26
Question
Q: how to thaw whole blood sample for Vitamin D BP ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-02-17
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Vitamin D BP for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2017-02-17
Question
Q: What is the optimal O.D. value for Vitamin D BP ELISA kit? I used your Vitamin D BP ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Vitamin D BP even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2015-11-11
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Vitamin D BP ELISA kit to have sensitivity of 150pg/ml, that means the minimum amount of Vitamin D BP that can be declared/interpreted as positive by the above standard is 150pg/ml.
Boster Scientific Support
Answered: 2015-11-11
