Mouse Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine®

Product Name

Mouse Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine®

View all Vitamin D BP ELISA kits

SKU/Catalog Number

EK2063

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Vitamin DBP ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse GC in cell culture supernatants, cell lysates, serum, plasma (heparin), saliva and urine. Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2063)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L17-S476

Sensitivity

<50 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Standard Dilution Instructions

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2063 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2063 is reactive to GC in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum, plasma (heparin), saliva and urine.

Application Guarantee

EK2063 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Vitamin D BP

Vitamin D-binding protein (DBP), also/originally known as gc-globulin (group-specific component), is a protein that in humans is encoded by the GC gene. It is mapped to 5 E1; 5 44.32 cM. The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Kit Components

Catalog Number	Description	Quantity
EK2063-CAP	Anti-Mouse GC Pre-coated 96-well strip microplate	1
EK2063-ST	Mouse GC Standard	2 vials, 10 ng/tube
EK2063-DA	Mouse GC Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.038	0.061	0.070	0.106	0.207	0.456	1.035	1.855

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Vitamin D BP, Dilution ratio of 1:50000, concentration in serum and plasma is around 286 ug/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Vitamin D BP in ELISA kits from four different production batches/lots.

Gene/Protein Information For GC (Source: Uniprot.Org, NCBI)

Alternative Names

DBP; DBP/GC; DBP-MAF; DBPVDBG; EC 6.3.1.5; Gc; gc-globulin; Gc-MAF; GRD3; group-specific component (vitamin D binding protein); Group-specific component; hDBP; VDB; VDBG; VDBP; Vitamin D BP; vitamin D-binding alpha-globulin; vitamin D-binding protein GC DBP, DBP-maf, DBP/GC, GRD3, Gc-MAF, GcMAF, HEL-S-51, VDB, VDBG, VDBP GC vitamin D binding protein vitamin D-binding protein|epididymis secretory protein Li 51|gc protein-derived macrophage activating factor|gc-globulin|group-specific component (vitamin D binding protein)|vitamin D-binding alpha-globulin|vitamin D-binding protein-macrophage activating factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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