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Product Info Summary
| SKU: | A00345-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-BAP1 Antibody Picoband®
SKU/Catalog Number
A00345-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BAP1 Antibody Picoband® catalog # A00345-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BAP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00345-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human BAP1 recombinant protein (Position: M1-E278).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00345-1 is reactive to BAP1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
88 kDa
Calculated molecular weight
26959 MW
Background of BAP1
BAP1, also known as BRCA1-associated protein-1, contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 is a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1 is expressed as a 4-kb mRNA in all human tissues, and mapped to 3p21.3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00345-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human 22RV1 whole cell, human MCF-7 whole cell, rat thymus tissue, rat kidney tissue, mouse kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of BAP1 using anti-BAP1 antibody (A00345-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 22RV1 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAP1 antigen affinity purified polyclonal antibody (Catalog # A00345-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAP1 at approximately 88KD. The expected band size for BAP1 is at 80KD.
Protein Target Info & Infographic
Gene/Protein Information For BAP1 (Source: Uniprot.org, NCBI)
Gene Name
BAP1
Full Name
Ubiquitin carboxyl-terminal hydrolase BAP1
Weight
26959 MW
Superfamily
peptidase C12 family
Alternative Names
Ubiquitin carboxyl-terminal hydrolase BAP1; BRCA1-associated protein 1; Cerebral protein 6; BAP1; KIAA0272; hucep-6 BAP1 HUCEP-13, UCHL2, hucep-6 BRCA1 associated protein 1 ubiquitin carboxyl-terminal hydrolase BAP1|BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)|cerebral protein 6|cerebral protein-13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAP1, check out the BAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BAP1 Antibody Picoband® (A00345-1)
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No publications found for A00345-1
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Customer Q&As
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16 Customer Q&As for Anti-BAP1 Antibody Picoband®
Question
Our lab used your anti-BAP1 antibody for ELISA on cervix carcinoma in the past. I am using mouse, and I plan to use the antibody for WB next. We are interested in examining cervix carcinoma as well as medulloblastoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
I have checked the website and datasheets of our anti-BAP1 antibody and it seems that A00345-1 has been tested on mouse in both ELISA and WB. Thus A00345-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-05
Question
I was wanting to use your anti-BAP1 antibody for ELISA for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
You can see on the product datasheet, A00345-1 anti-BAP1 antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-10
Question
Will A00345-1 anti-BAP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
It shows on the product datasheet, A00345-1 anti-BAP1 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-31
Question
We are interested in using your anti-BAP1 antibody for uch proteinases studies. Has this antibody been tested with western blotting on hepg2 whole cell lysates? We would like to see some validation images before ordering.
M. Baker
Verified customer
Asked: 2019-10-22
Answer
We appreciate your inquiry. This A00345-1 anti-BAP1 antibody is validated on human hela, hela whole cell lysates, hepg2 whole cell lysates, 22rv1 whole cell lysates, rat thymus tissue, kidney tissue, mouse kidney tissue. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-22
Question
I am interested in to test anti-BAP1 antibody A00345-1 on rat brain for research purposes, then I may be interested in using anti-BAP1 antibody A00345-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
The products we sell, including anti-BAP1 antibody A00345-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-10
Question
Please see the WB image, lot number and protocol we used for brain using anti-BAP1 antibody A00345-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-21
Question
Do you have a BSA free version of anti-BAP1 antibody A00345-1 available?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-BAP1 antibody A00345-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-BAP1 antibody A00345-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-04-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-08
Question
I see that the anti-BAP1 antibody A00345-1 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-11
Question
Can you help my question with product A00345-1, anti-BAP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00345-1 anti-BAP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-05
Question
We are currently using anti-BAP1 antibody A00345-1 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
The anti-BAP1 antibody (A00345-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-28
Question
My team were well pleased with the WB result of your anti-BAP1 antibody. However we have observed positive staining in leukemic t-cell cytoplasm using this antibody. Is that expected? Could you tell me where is BAP1 supposed to be expressed?
S. Krishna
Verified customer
Asked: 2017-04-12
Answer
According to literature, leukemic t-cell does express BAP1. Generally BAP1 expresses in cytoplasm. Regarding which tissues have BAP1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9528852
Brain, Pubmed ID: 9039502
Cervix carcinoma, Pubmed ID: 18220336, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Medulloblastoma, Pubmed ID: 12800201
Skin, Pubmed ID: 15489334
Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-04-12
Question
We have seen staining in mouse testis. Any tips? Is anti-BAP1 antibody supposed to stain testis positively?
R. Miller
Verified customer
Asked: 2016-05-24
Answer
From what I have seen in literature testis does express BAP1. From what I have seen in Uniprot.org, BAP1 is expressed in testis, b-cell, brain, skin, medulloblastoma, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have BAP1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9528852
Brain, Pubmed ID: 9039502
Cervix carcinoma, Pubmed ID: 18220336, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Medulloblastoma, Pubmed ID: 12800201
Skin, Pubmed ID: 15489334
Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-05-24
Question
Is a blocking peptide available for product anti-BAP1 antibody (A00345-1)?
A. Wu
Verified customer
Asked: 2015-09-29
Answer
We do provide the blocking peptide for product anti-BAP1 antibody (A00345-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-09-29
Question
Will anti-BAP1 antibody A00345-1 work for ELISA with brain?
W. Li
Verified customer
Asked: 2013-11-14
Answer
According to the expression profile of brain, BAP1 is highly expressed in brain. So, it is likely that anti-BAP1 antibody A00345-1 will work for ELISA with brain.
Boster Scientific Support
Answered: 2013-11-14
Question
Is this A00345-1 anti-BAP1 antibody reactive to the isotypes of BAP1?
C. Williams
Verified customer
Asked: 2013-07-18
Answer
The immunogen of A00345-1 anti-BAP1 antibody is E. coli-derived human BAP1 recombinant protein (Position: M1-E278). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-07-18
