Product Info Summary
SKU: | A30654 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-RPC8 Antibody
SKU/Catalog Number
A30654
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RPC8 Antibody (Catalog# A30654). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPC8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30654)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RPC8. Immunogen sequence location: 151-200
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30654 is reactive to POLR3H in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
22918 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30654 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from K562 cells, using RPC8 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For POLR3H (Source: Uniprot.org, NCBI)
Gene Name
POLR3H
Full Name
DNA-directed RNA polymerase III subunit RPC8
Weight
22918 MW
Superfamily
eukaryotic RPB7/RPC8 RNA polymerase subunit family
Alternative Names
POLR3H;KIAA1665;RPC8;DNA-directed RNA polymerase III subunit RPC8;RNA polymerase III subunit C8;DNA-directed RNA polymerase III subunit H;RNA polymerase III subunit 22.9 kDa subunit;RPC22.9 Polr3h|5031409G22Rik, RP, RPC8|polymerase (RNA) III (DNA directed) polypeptide H|DNA-directed RNA polymerase III subunit RPC8|DNA-directed RNA polymerase III subunit H|RNA polymerase III subunit C7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR3H, check out the POLR3H Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR3H: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPC8 Antibody (A30654)
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No publications found for A30654
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1 Customer Q&As for Anti-RPC8 Antibody
Question
We are currently using anti-RPC8 antibody A30654 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
K. Mangal
Verified customer
Asked: 2013-11-12
Answer
The anti-RPC8 antibody (A30654) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-12