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Product Info Summary
| SKU: | A00722-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Rock-1 Antibody
SKU/Catalog Number
A00722-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Rock-1 Antibody catalog # A00722-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rock-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00722-3)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Rock-1. AA range:262-311
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00722-3 is reactive to ROCK1 in Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
158175 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00722-3 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ICC 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Figure 10. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of mouse testis tissue. A00722-3 was diluted at 1:200 (4°C
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Figure 11. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of rat brain tissue. A00722-3 was diluted at 1:200 (4°C
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Figure 12. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of rat heart tissue. A00722-3 was diluted at 1:200 (4°C
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Figure 13. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of rat kidney tissue. A00722-3 was diluted at 1:200 (4°C
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Figure 14. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of rat lung tissue. A00722-3 was diluted at 1:200 (4°C
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Figure 15. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of rat spinal cord tissue. A00722-3 was diluted at 1:200 (4°C
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Figure 16. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of rat spleen tissue. A00722-3 was diluted at 1:200 (4°C
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Figure 17. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of rat testis tissue. Anti-Cleaved-Caspase-1 (D210) antibody was diluted at 1:200 (4°C
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Figure 2. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of human-liver-cancer tissue. Anti-Rock-1 at 1:200 (4°C
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Figure 3. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of human lung cancer tissue. Anti-Rock-1 at 1:200 (4°C
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Figure 4. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of human stomach cancer tissue. Anti-Rock-1 at 1:200 (4°C
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Figure 5. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of human tonsil tissue. Anti-Rock-1 at 1:200 (4°C
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Figure 6. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of mouse kidney tissue. Anti-Rock-1 at 1:200 (4°C
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Figure 7. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of mouse liver tissue. Anti-Rock-1 at 1:200 (4°C
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Figure 8. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of mouse lung tissue. A00722-3 was diluted at 1:200 (4°C
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Figure 9. Immunohistochemistry validation of ROCK1 using Anti-Rock-1 Antibody (A00722-3).
Immunohistochemical analysis of mouse spleen tissue. A00722-3 was diluted at 1:200 (4°C
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Figure 1. Western blotting validation for Anti-Rock-1 Antibody A00722-3
Western blot (WB) analysis of Rock-1 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ROCK1 (Source: Uniprot.org, NCBI)
Gene Name
ROCK1
Full Name
Rho-associated protein kinase 1
Weight
158175 MW
Superfamily
protein kinase superfamily
Alternative Names
Rho-associated protein kinase 1;2.7.11.1;Renal carcinoma antigen NY-REN-35;Rho-associated, coiled-coil-containing protein kinase 1;Rho-associated, coiled-coil-containing protein kinase I;ROCK-I;p160 ROCK-1;p160ROCK;ROCK1; ROCK1 P160ROCK, ROCK-I Rho associated coiled-coil containing protein kinase 1 rho-associated protein kinase 1|p160 ROCK-1|renal carcinoma NY-REN-35
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ROCK1, check out the ROCK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ROCK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rock-1 Antibody (A00722-3)
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