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Product Info Summary
| SKU: | M00722 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ROCK1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00722
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ROCK1 Rabbit Monoclonal Antibody catalog # M00722. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ROCK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00722)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CDE-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ROCK1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00722 is reactive to ROCK1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
170 kDa
Calculated molecular weight
158175 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00722 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human U2OS whole cell, human 293T whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
IHC: human kidney tissue, Rat stomach tissue, Rat heart tissue, Human squamous carcinoma tissue, Human esophageal carcinoma tissue, Mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FROCK1 using anti-ROCK1 antibody (M00722).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK1 antigen affinity purified monoclonal antibody (Catalog # M00722) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK1 at approximately 170 kDa. The expected band size for ROCK1 is at 158 kDa.
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Immunohistochemical analysis of paraffin-embedded human kidney, using ROCK1 Antibody(M00722)
ROCK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ROCK1 Antibody (M00722)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:150 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody at 1:150 dilution.
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Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using the Antibody at 1:150 dilution.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:150 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse heart, using the Antibody at 1:150 dilution.
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Immunofluorescent analysis of Hela cells, using ROCK1 Antibody .
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For ROCK1 (Source: Uniprot.org, NCBI)
Gene Name
ROCK1
Full Name
Rho-associated protein kinase 1
Weight
158175 MW
Superfamily
protein kinase superfamily
Alternative Names
Rho-associated protein kinase 1;2.7.11.1;Renal carcinoma antigen NY-REN-35;Rho-associated, coiled-coil-containing protein kinase 1;Rho-associated, coiled-coil-containing protein kinase I;ROCK-I;p160 ROCK-1;p160ROCK;ROCK1; ROCK1 P160ROCK, ROCK-I Rho associated coiled-coil containing protein kinase 1 rho-associated protein kinase 1|p160 ROCK-1|renal carcinoma NY-REN-35
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ROCK1, check out the ROCK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ROCK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ROCK1 Rabbit Monoclonal Antibody (M00722)
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Customer Q&As
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6 Customer Q&As for Anti-ROCK1 Rabbit Monoclonal Antibody
Question
Is this M00722 anti-ROCK1 Rabbit Monoclonal antibody reactive to the isotypes of ROCK1?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
The immunogen of M00722 anti-ROCK1 Rabbit Monoclonal antibody is A synthesized peptide derived from human ROCK1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-14
Question
We are interested in to test anti-ROCK1 Rabbit Monoclonal antibody M00722 on rat leukemic t-cell for research purposes, then I may be interested in using anti-ROCK1 Rabbit Monoclonal antibody M00722 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-26
Answer
The products we sell, including anti-ROCK1 Rabbit Monoclonal antibody M00722, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-26
Question
We are currently using anti-ROCK1 Rabbit Monoclonal antibody M00722 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
The anti-ROCK1 Rabbit Monoclonal antibody (M00722) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-16
Question
Does anti-ROCK1 Rabbit Monoclonal antibody M00722 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2017-09-01
Answer
According to the expression profile of leukemic t-cell, ROCK1 is highly expressed in leukemic t-cell. So, it is likely that anti-ROCK1 Rabbit Monoclonal antibody M00722 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2017-09-01
Question
I was wanting to use your anti-ROCK1 Rabbit Monoclonal antibody for IHC for rat leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukemic t-cell identification?
J. Zhang
Verified customer
Asked: 2017-04-21
Answer
It shows on the product datasheet, M00722 anti-ROCK1 Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-21
Question
Does anti-ROCK1 Rabbit Monoclonal antibody M00722 work on horse IHC with popliteal artery?
J. Krishna
Verified customer
Asked: 2013-03-19
Answer
Our lab technicians have not tested anti-ROCK1 Rabbit Monoclonal antibody M00722 on horse. You can run a BLAST between horse and the immunogen sequence of anti-ROCK1 Rabbit Monoclonal antibody M00722 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse popliteal artery in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-03-19
