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Product Info Summary
| SKU: | M00524 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ROC1 Monoclonal Antibody
SKU/Catalog Number
M00524
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ROC1 Monoclonal Antibody catalog # M00524. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ROC1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00524)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADCI-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ROC1 E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00524 is reactive to RBX1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40 kDa
Calculated molecular weight
94135 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00524 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:100
Positive Control
WB: MCF7 cell
Validation Images & Assay Conditions
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Western blot analysis of ROC1 expression in MCF7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RBX1 (Source: Uniprot.org, NCBI)
Gene Name
RBX1
Full Name
E3 ubiquitin-protein ligase RBX1
Weight
94135 MW
Superfamily
RING-box family
Alternative Names
Signal transducer and activator of transcription 6;IL-4 Stat;STAT6; RBX1 BA554C12.1, RNF75, ROC1 ring-box 1 E3 ubiquitin-protein ligase RBX1|E3 ubiquitin-protein transferase RBX1|RING finger protein 75|RING-box protein 1|ZYP protein|regulator of cullins 1|ring-box 1, E3 ubiquitin protein ligase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RBX1, check out the RBX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RBX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ROC1 Monoclonal Antibody (M00524)
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