RBX1 Antibodies

E3 ubiquitin-protein ligase RBX1 (RBX1)

E3 ubiquitin ligase component of multiple cullin-RING- based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription- coupled nucleotide excision repair (PubMed:10230407, PubMed:10579999, PubMed:15983046, PubMed:16678110, PubMed:19112177, PubMed:19679664, PubMed:23455478, PubMed:27565346, PubMed:29769719, PubMed:11961546, PubMed:22748924). CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (PubMed:27565346).

The functional specificity of the E3 ubiquitin-protein ligase complexes depends upon the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation.

Gene Information

Human
Gene Name:	RBX1
Uniprot:	P62877
Entrez:	9978

Family

Belongs to:
RING-box family

Alternative Names

BA554C12.1; EC 6.3.2.-; FLJ60363; MGC13357; Protein ZYP; RBX1; Regulator of cullins 1; Ring Box 1; RING box protein 1; RING finger protein 75; ring-box 1; ring-box 1, E3 ubiquitin protein ligase; RING-box protein 1; RNF75; RNF75MGC1481; ROC1E3 ubiquitin-protein ligase RBX1; ZYP Protein

Molecular Weight

Mass (kDA):
12.274 kDA

Genomic Context

Human
Location:	22q13.2
Sequence:	22; NC_000022.11 (40951378..40973309)

Tissue Specificity

Widely expressed.

Subcellular Localizations

Cytoplasm. Nucleus.

Diseases

• Neoplasms
• Malignant Neoplasms
• Von Hippel-lindau Syndrome
• Cell Transformation, Neoplastic
• Carcinoma
• Renal Cell Carcinoma
• Hypoxia
• Kidney Neoplasm
• Mammary Neoplasms
• Leukemia
• Adenovirus Infections
• Retinoblastoma
• Growth Arrest

• Fetal Growth Retardation
• Immunologic Deficiency Syndromes
• Adenocarcinoma
• Liver Carcinoma
• Protrusion
• Malignant Neoplasm Of Kidney
• Hyperplasia

Interacting Proteins

• CUL1
• CUL2
• CUL4B
• CUL5
• GLMN

• SESN2
• SKP2
• UBE2M

Pathways

• Cell Cycle
• Conjugation
• Cell Proliferation
• Proteolysis
• Cell Growth
• Dna Replication
• Rna Interference
• Localization
• Senescence
• Cell Death
• S Phase
• Protein Ubiquitination
• Nuclear Export

• Pathogenesis
• Double-strand Break Repair
• Translation
• Response To Hypoxia
• Viral Replication
• Cell Adhesion
• Embryo Development

PTMs

• Ubiquitination
• Phosphorylation
• Cleavage

• Sumoylation
• Reduction
• Methylation

• Dephosphorylation

Research Areas

• Cancer
• Cell Cycle and Replication
• Hypoxia
• Neuroscience
• Wnt Signaling Pathway

• Zinc Finger

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RBX1

Boster Bio: Best Uses Of The RBX1 Marker

This article will explain the advantages of RBX1 antibodies and the advantages associated with its product range. This article will also discuss the validation and customization options for the product. Boster's products are validated using confirmed negative and positive samples to ensure high specificity and affluence. Boster also rewards the first reviewer with credits for their products. This program benefits all scientists from around the world.

Anti-RBX1 Marker

Boster Bio's Anti-RBX1 marker is a mouse-specific anti-body that reacts with human recombinant protein that spans 108 amino acids. It is provided in PBS with 0.09 percent sodium azide. The antibody is purified using the protein G column and dialysis using PBS. It was made from a mouse immunized with human recombinant protein.

The antibody was developed by researchers from Florida State University and the University of South Carolina. J. Thomas Parsons, PhD and Amy Bouton (PhD) were also collaborators. Robert B. Meagher, PhD and Rima R. Roseberg PhD, PhD have contributed antibodies to ferroportin and mCherry.

Another antibody used in the study was an anti-Nedd8 antibody. This antibody was used in Western blot analysis as a primary antibody. It deneddylates Cul-1. Thus, the study proves that Jab1 deneddylates Cul-1 in the mouse brain as well as liver. It also suggests that the anti-Nedd8 antibody is effective in the detection of Cul-1.

Other researchers who contributed to the study are Ronald P. Taylor, PhD and Gail M. Seigel, PhD, of the Massachusetts General Hospital. They also contributed additional Plant Carbohydrate antibodies , as well as the Human S100A8/A9 heteromer. These contributions have enabled this research. Multiple studies with human mRNA have confirmed the Anti-RBX1 Marker of Boster Bio.

Validation

The RBX1 gene is an essential regulator of the neddylation process. The neddylation process involves modification of target proteins using an enzyme that is conserved, NAE1-UBA3 heteromer. This enzyme, RBX1, is involved in the activation of POLR2A through K63-linked Ubiquitination. It also aids in the stability of the RNAP2 protein, which is crucial for mitochondrial biogenesis.

Product range

Boster Bio the RBX1 product line was designed to detect RBX1 within human tissues and samples. The company's antibody kits are available for various applications that include the fields of developmental and cancer biology. Boster Bio's ELISA kits are extremely sensitive, with sensitiveness as high as picograms. Boster's antibodies can detect the RBX1 gene, which is a potential candidate for gene therapy. Boster's ELISA kit has been validated against a panel consisting of 250 human cells and tissues. The sensitivity and specificity of Boster's antibodies is high, resulting in a high affinity.

This RBX1 antibody is produced by mouse immunized with human recombinant proteins that contain 1-108 amino acids. It is delivered in PBS containing 0.09% sodium azide, and has been purified by dialysis on PBS. The Boster Bio RBX1 antibody is derived from mice immunized with human proteins recombinant with a C-Jun activation domain (CSN).

BeNeLux deliveries

Flow Cytometry is a technique that utilizes cells as samples to determine biological markers. Boster offers high affinity primary antibodies that are monoclonal as well as polyclonal. Boster has also achieved a the highest citation rates throughout their 25-year history. They are also available in customized solutions for custom research and BeNeLux deliveries. Learn more about the benefits of Boster's RBX1 markers.

Research antibody kits and ELISA kits are available through Boster Bio. Boster Bio has validated its antibodies against 250 cell tissues and lines to ensure high specificity and adsorption. Boster credits the first reviewers who have evaluated their products. This program is open for researchers from all over the world. The best applications of the RBX1 mark