Anti-RBX1 Antibody

Product Name

Anti-RBX1 Antibody

View all RBX1 Antibodies

SKU/Catalog Number

M00524-1

Size

50 µl

Description

Boster Bio Anti-RBX1 Antibody (Catalog # M00524-1). Tested in WB application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-RBX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00524-1)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1859CT866.7.47

Isotype

IgG1,κ

Immunogen

This antibody is generated from a mouse immunized with a recombinant protein between 1-108 amino acids from human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00524-1 is reactive to RBX1 in Human, Mouse

Applications

M00524-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

12274 Da

Background of RBX1

E3 ubiquitin ligase component of multiple cullin-RING- based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000

Validation Images & Assay Conditions

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All lanes : Anti-RBX1 at dilution
Lane 1: Jurkat whole cell lysate
Lane 2: HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 12kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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All lanes : Anti-RBX1 at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 12 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For RBX1 (Source: Uniprot.org, NCBI)

Alternative Names

BA554C12.1; EC 6.3.2.-; FLJ60363; MGC13357; Protein ZYP; RBX1; Regulator of cullins 1; Ring Box 1; RING box protein 1; RING finger protein 75; ring-box 1; ring-box 1, E3 ubiquitin protein ligase; RING-box protein 1; RNF75; RNF75MGC1481; ROC1E3 ubiquitin-protein ligase RBX1; ZYP Protein RBX1 BA554C12.1, RNF75, ROC1 ring-box 1 E3 ubiquitin-protein ligase RBX1|E3 ubiquitin-protein transferase RBX1|RING finger protein 75|RING-box protein 1|ZYP protein|regulator of cullins 1|ring-box 1, E3 ubiquitin protein ligase

For more info on RBX1, check out the RBX1 Infographic

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In this infographic, you will see the following information for RBX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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