Anti-ROBO1 Antibody Picoband®

ROBO1 antibody

Boster Bio Anti-ROBO1 Antibody Picoband® catalog # A01530-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A01530-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, WB

Product Name

Anti-ROBO1 Antibody Picoband®

View all ROBO1 Antibodies

SKU/Catalog Number

A01530-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ROBO1 Antibody Picoband® catalog # A01530-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ROBO1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01530-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ROBO1 recombinant protein (Position: F264-P897).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01530-2 is reactive to ROBO1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

250 kDa

Calculated molecular weight

180,930 MW

Background of ROBO1

Roundabout homolog 1 is a protein that in humans is encoded by the ROBO1 gene. Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01530-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml

Positive Control

WB: human HepG2 whole cell, human Hela whole cell, human 293T whole cell, rat brain tissue, mouse brain tissue
FCM: A549 cell

Validation Images & Assay Conditions

Gene/Protein Information For ROBO1 (Source: Uniprot.org, NCBI)

Gene Name

ROBO1

Full Name

Roundabout homolog 1

Weight

180,930 MW

Superfamily

immunoglobulin superfamily

Alternative Names

Roundabout homolog 1; Deleted in U twenty twenty; H-Robo-1; ROBO1; DUTT1 ROBO1 DUTT1, SAX3 roundabout guidance receptor 1 roundabout homolog 1|deleted in U twenty twenty|roundabout, axon guidance receptor, homolog 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ROBO1, check out the ROBO1 Infographic

ROBO1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ROBO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-ROBO1 Antibody Picoband®

Question

We are currently using anti-ROBO1 antibody A01530-2 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

The anti-ROBO1 antibody (A01530-2) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-16

Order DetailsPrice
A01530-2

100μg

$370
A01530-2-10ug

10μg sample (liquid)

$99
A01530-2-Biotin

100 μg Biotin conjugated

$570
A01530-2-Cy3

100 μg Cy3 conjugated

$570
A01530-2-Dylight488

100 μg Dylight488 conjugated

$570
A01530-2-Dylight550

100 μg Dylight550 conjugated

$570
A01530-2-Dylight594

100 μg Dylight594 conjugated

$570
A01530-2-FITC

100 μg FITC conjugated

$570
A01530-2-HRP

100 μg HRP conjugated

$570
A01530-2-APC

100 μg APC conjugated

$670
A01530-2-PE

100 μg PE conjugated

$670
A01530-2-iFluor647

100 μg iFluor647 conjugated

$670
A01530-2-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A01530-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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