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Anti-Ran Antibody Picoband® (monoclonal, 5D5)

Name: Anti-Ran Antibody Picoband® (monoclonal, 5D5)
Brand: Boster Bio
SKU: M00204-1
Price: 370 USD
Availability: InStock
Rating: 100 (8 reviews)

RAN antibody

Boster Bio Anti-Ran Antibody Picoband® (monoclonal, 5D5) catalog # M00204-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU:	M00204-1
Size:	100 μg/vial
Reactive Species:	Human, Mouse, Rat
Host:	Mouse
Application:	Flow Cytometry, IF, ICC, WB

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Product info

Product Name

Anti-Ran Antibody Picoband® (monoclonal, 5D5)

View all RAN Antibodies

SKU/Catalog Number

M00204-1

Size

100 μg/vial

Form

Lyophilized

Description

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ran Antibody Picoband® (monoclonal, 5D5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00204-1)

Host

Mouse

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Monoclonal

Clone Number

5D5

Isotype

Mouse IgG2b

Immunogen

E. coli-derived human Ran recombinant protein (Position: A2-L216). Human Ran shares 100% amino acid (aa) sequence identity with both mouse and rat Ran.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00204-1 is reactive to RAN in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Observed Molecular Weight

24 kDa

Calculated molecular weight

24.423kDa

Background of RAN

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Application & Images

Applications

M00204-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

Positive Control

WB: human HL-60 whole cell, human T-47D whole cell, human A549 whole cell, human U2OS whole cell, human THP-1 whole cell, human HepG2 whole cell, human PANC-1 whole cell, human SW620 whole cell,, rat testis tissue, mouse lung tissue, mouse kidney tissue, mouse testis tissue, mouse Neuro-2a whole cell,
ICC/IF: U20S cell
FCM: PC-3 cell

Validation Images & Assay Conditions

m00204 1 ran primary antibodies wb testing 1

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Figure 1. Western blot analysis of Ran using anti-Ran antibody (M00204-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: human THP-1 whole cell lysates,
Lane 6: human HepG2 whole cell lysates,
Lane 7: human PANC-1 whole cell lysates,
Lane 8: human SW620 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Ran antigen affinity purified monoclonal antibody (Catalog # M00204-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Ran at approximately 24KD. The expected band size for Ran is at 24KD.

m00204 1 ran primary antibodies wb testing 2

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Figure 2. Western blot analysis of Ran using anti-Ran antibody (M00204-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: mouse kidney tissue lysates,
Lane 4: mouse testis tissue lysates,
Lane 5: mouse Neuro-2a whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Ran antigen affinity purified monoclonal antibody (Catalog # M00204-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Ran at approximately 24KD. The expected band size for Ran is at 24KD.

m00204 1 ran primary antibodies fc testing 3

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Figure 3. Flow Cytometry analysis of PC-3 cells using anti-Ran antibody (M00204-1).
Overlay histogram showing PC-3 cells stained with M00204-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ran Antibody (M00204-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

m00204 1 ran primary antibodies if testing 4

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Figure 4. IF analysis of Ran using anti-Ran antibody (M00204-1).
Ran was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Ran Antibody (M00204-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Protein Target Info & Infographic

Gene/Protein Information For RAN (Source: Uniprot.org, NCBI)

Gene Name

RAN

Full Name

GTP-binding nuclear protein Ran

Weight

24.423kDa

Superfamily

small GTPase superfamily

Alternative Names

GTP-binding nuclear protein Ran; Androgen receptor-associated protein 24; GTPase Ran; Ras-like protein TC4; Ras-related nuclear protein; RAN; ARA24; OK/SW-cl.81 RAN ARA24, Gsp1, TC4 RAN, member RAS oncogene family GTP-binding nuclear protein Ran|GTPase Ran|RanGTPase|androgen receptor-associated protein 24|guanosine triphosphatase Ran|member RAS oncogene family|ras-like protein TC4|ras-related nuclear protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RAN, check out the RAN Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RAN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Specific Publications For Anti-Ran Antibody Picoband® (monoclonal, 5D5) (M00204-1)

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6 Customer Q&As for Anti-Ran Antibody Picoband® (monoclonal, 5D5)

Question

you antibody using your anti-Ran antibody (monoclonal, 5D5) for pre-mirna export from nucleus studies. Has this antibody been tested with western blotting on mouse lung tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

Thank you for your inquiry. This M00204-1 anti-Ran antibody (monoclonal, 5D5) is tested on human a549, a549 whole cell lysates, u2os whole cell lysates, hepg2 whole cell lysates, sw620 whole cell lysates, mouse lung tissue, rat testis tissue, kidney tissue. It is guaranteed to work for Flow Cytometry, IF, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-07

Question

Free Secondary Antibody

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M00204-1

Promotion :

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)

$370.00

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Anti-Ran Antibody Picoband® (monoclonal, 5D5)

RAN antibody