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Product Info Summary
| SKU: | A04017 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Glycine receptor subunit beta GLRB Antibody
SKU/Catalog Number
A04017
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Glycine receptor subunit beta GLRB Antibody (Catalog # A04017). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glycine receptor subunit beta GLRB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04017)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Synthesized peptide derived from internal of human GLRB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04017 is reactive to GLRB in Human
Reconstitution
Calculated molecular weight
56122 MW
Background of GLRB
The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
Handford C.A., Brain Res. Mol. Brain Res. 35:211-219(1996).
Milani N., Genomics 50:341-345(1998).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04017 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from A549 cells and K562 cells, using GLRB antibody A04017.
Protein Target Info & Infographic
Gene/Protein Information For GLRB (Source: Uniprot.org, NCBI)
Gene Name
GLRB
Full Name
Glycine receptor subunit beta
Weight
56122 MW
Superfamily
ligand-gated ion channel (TC 1.A.9) family
Alternative Names
Glycine receptor subunit beta;Glycine receptor 58 kDa subunit;GLRB; GLRB HKPX2 glycine receptor beta glycine receptor subunit beta|glycine receptor 58 kDa subunit|glycine receptor, beta subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GLRB, check out the GLRB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GLRB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glycine receptor subunit beta GLRB Antibody (A04017)
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No publications found for A04017
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Glycine receptor subunit beta GLRB Antibody
Question
Would anti-GLRB antibody A04017 work for WB with brain?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
According to the expression profile of brain, GLRB is highly expressed in brain. So, it is likely that anti-GLRB antibody A04017 will work for WB with brain.
Boster Scientific Support
Answered: 2020-02-12
Question
I have a question about product A04017, anti-GLRB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04017 anti-GLRB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-10
Question
We are currently using anti-GLRB antibody A04017 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
C. Dhar
Verified customer
Asked: 2019-03-28
Answer
The anti-GLRB antibody (A04017) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-28
Question
Will A04017 anti-GLRB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-06-08
Answer
You can see on the product datasheet, A04017 anti-GLRB antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-08
Question
We want to test anti-GLRB antibody A04017 on human brain for research purposes, then I may be interested in using anti-GLRB antibody A04017 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-04-03
Answer
The products we sell, including anti-GLRB antibody A04017, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-03
Question
I was wanting to use your anti-GLRB antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2018-02-19
Answer
As indicated on the product datasheet, A04017 anti-GLRB antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-19
Question
See below the WB image, lot number and protocol we used for brain using anti-GLRB antibody A04017. Please let me know if you require anything else.
A. Evans
Verified customer
Asked: 2014-10-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-03
