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Product Info Summary
| SKU: | A06979-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-RNF40 Antibody
SKU/Catalog Number
A06979-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RNF40 Antibody catalog # A06979-1. Tested in WB,IP,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RNF40 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06979-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein within human RNF40 aa 200-400.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06979-1 is reactive to RNF40 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
113678 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06979-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
IP: 1:10-1:50
IHC: 1:50
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of RNF40 using Anti-RNF40 Antibody (A06979-1).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RNF40 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins. The tissues were blocked in 5% BSA for 30 minutes at room temperature
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Figure 1. Western blotting validation for Anti-RNF40 Antibody A06979-1
Western blot analysis of RNF40 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG -HRP Secondary Antibody (HA1001) at 1:5Protein Target Info & Infographic
Gene/Protein Information For RNF40 (Source: Uniprot.org, NCBI)
Gene Name
RNF40
Full Name
E3 ubiquitin-protein ligase BRE1B
Weight
113678 MW
Superfamily
BRE1 family
Alternative Names
E3 ubiquitin-protein ligase BRE1B;BRE1-B;2.3.2.27 RNF40 BRE1B, RBP95, STARING ring finger protein 40 E3 ubiquitin-protein ligase BRE1B|95 kDa retinoblastoma protein binding protein|95 kDa retinoblastoma-associated protein|BRE1 E3 ubiquitin ligase homolog B|BRE1-B|RING-type E3 ubiquitin transferase BRE1B|Rb-associated protein|ring finger protein 40, E3 ubiquitin protein ligase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF40, check out the RNF40 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF40: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNF40 Antibody (A06979-1)
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