Anti-Phospho-eEF1A2 (Ser358) Antibody

Product Name

Anti-Phospho-eEF1A2 (Ser358) Antibody

View all EEF1A2 Antibodies

SKU/Catalog Number

P03304

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-eEF1A2 (Ser358) Antibody (Catalog # P03304). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-eEF1A2 (Ser358) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03304)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P03304 is reactive to EEF1A2 in Human, Mouse

Reconstitution

Observed Molecular Weight

52 kDa

Calculated molecular weight

50470 MW

Background of EEF1A2

Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle (Knudsen et al., 1993). Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors (Anand et al., 2002). Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites (Soares et al., 2009). Ribosome-associated JNK phosphorylates Ser-358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome (Gandin et al., 2013).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

P03304 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For EEF1A2 (Source: Uniprot.org, NCBI)

Alternative Names

eEF1A-2 antibody, Eef1a2 antibody, EEF1AL antibody, EF 1 alpha 2 antibody, EF-1-alpha-2 antibody, EF1A antibody, EF1A2_HUMAN antibody, Elongation factor 1 A 2 antibody, Elongation factor 1 alpha antibody, Elongation factor 1 alpha 2 antibody, Elongation factor 1-alpha 2 antibody, Eukaryotic elongation factor 1 A-2 antibody, Eukaryotic translation elongation factor 1 alpha 2 antibody, FLJ41696 antibody, HS1 antibody, OTTHUMP00000031776 antibody, Statin like antibody, Statin S1 antibody, Statin-S1 antibody, STN antibody, STNL antibody EEF1A2 DEE33, EEF1AL, EF-1-alpha-2, EF1A, EIEE33, HS1, MRD38, STN, STNL eukaryotic translation elongation factor 1 alpha 2 elongation factor 1-alpha 2|eukaryotic elongation factor 1 A-2|statin-S1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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