Anti-RNF20 Mouse Monoclonal Antibody [Clone ID: OTI2B9]

Product Name

Anti-RNF20 Mouse Monoclonal Antibody [Clone ID: OTI2B9]

View all RNF20 Antibodies

SKU/Catalog Number

M03457-3

Size

100 µl

Description

Boster Bio RNF20 mouse monoclonal antibody,clone OTI2B9. Catalog# M03457-3. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C as received.

Cite This Product

Anti-RNF20 Mouse Monoclonal Antibody [Clone ID: OTI2B9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03457-3)

Host

Mouse

Contents

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

OTI2B9

Isotype

IgG2b

Immunogen

Human recombinant protein fragment corresponding to amino acids 493-754 of human RNF20 (NP_062538) produced in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03457-3 is reactive to RNF20 in Human, Mouse, Rat

Applications

M03457-3 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Validation Images & Assay Conditions

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF20 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF20 (1:500).

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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RNF20 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min

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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RNF20 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min

Gene/Protein Information For RNF20 (Source: Uniprot.org, NCBI)

Alternative Names

BRE1 E3 ubiquitin ligase homolog; BRE1; BRE1-A; BRE1AMGC129667; E3 ubiquitin-protein ligase BRE1A; EC 6.3.2; EC 6.3.2.-; FLJ11189; FLJ20382; hBRE1MGC129668; homolog of S. cerevisiae BRE1; KAIA2779; ring finger protein 20KIAA2779 RNF20 BRE1, BRE1A, hBRE1 ring finger protein 20 E3 ubiquitin-protein ligase BRE1A|BRE1 E3 ubiquitin ligase homolog|RING-type E3 ubiquitin transferase BRE1A|homolog of S. cerevisiae BRE1

For more info on RNF20, check out the RNF20 Infographic

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In this infographic, you will see the following information for RNF20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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