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Product Info Summary
| SKU: | M03457-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-RNF20 Antibody
SKU/Catalog Number
M03457-2
Size
50 µl
Description
Boster Bio Anti-RNF20 Antibody (Catalog # M03457-2). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RNF20 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03457-2)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1594CT552.128.36
Isotype
IgG1,k
Immunogen
This RNF20 antibody is generated from a mouse immunized with a recombinant protein of human RNF20.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03457-2 is reactive to RNF20 in Human
Applications
M03457-2 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
113662 Da
Background of RNF20
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-RNF20 Antibody at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: HL-60 whole cell lysate
Lane 4: U87-MG whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 114 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For RNF20 (Source: Uniprot.org, NCBI)
Gene Name
RNF20
Full Name
E3 ubiquitin-protein ligase BRE1A
Weight
113662 Da
Superfamily
BRE1 family
Alternative Names
BRE1 E3 ubiquitin ligase homolog; BRE1; BRE1-A; BRE1AMGC129667; E3 ubiquitin-protein ligase BRE1A; EC 6.3.2; EC 6.3.2.-; FLJ11189; FLJ20382; hBRE1MGC129668; homolog of S. cerevisiae BRE1; KAIA2779; ring finger protein 20KIAA2779 RNF20 BRE1, BRE1A, hBRE1 ring finger protein 20 E3 ubiquitin-protein ligase BRE1A|BRE1 E3 ubiquitin ligase homolog|RING-type E3 ubiquitin transferase BRE1A|homolog of S. cerevisiae BRE1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF20, check out the RNF20 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNF20 Antibody (M03457-2)
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