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Product Info Summary
| SKU: | A15709-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-RNF166 Antibody (Center)
SKU/Catalog Number
A15709-1
Size
80 µl
Description
Boster Bio Anti-RNF166 Antibody (Center) (Catalog # A15709-1). Tested in IF, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RNF166 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15709-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RNF166 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-97 amino acids from the Central region of human RNF166.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A15709-1 is reactive to RNF166 in Human, Mouse
Reconstitution
Calculated molecular weight
26122 Da
Background of Rnf166
Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66% amino acid sequence identity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15709-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
Validation Images & Assay Conditions
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Fluorescent image of NIH/3T3 cells stained with RNF166 Antibody (Center). A15709-1 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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RNF166 Antibody (Center) western blot analysis in 293,mouse NIH/3T3 cell line and testis tissue lysates (35ug/lane).This demonstrates the RNF166 antibody detected the RNF166 protein (arrow).
Protein Target Info & Infographic
Gene/Protein Information For RNF166 (Source: Uniprot.org, NCBI)
Gene Name
RNF166
Full Name
E3 ubiquitin-protein ligase RNF166
Weight
26122 Da
Alternative Names
RING finger protein 166, RNF166
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF166, check out the RNF166 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF166: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNF166 Antibody (Center) (A15709-1)
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