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Product Info Summary
| SKU: | A07386 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-RNAse H2A Antibody
SKU/Catalog Number
A07386
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RNAse H2A Antibody (Catalog # A07386). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RNAse H2A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RNAse H2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07386)
Host
Rabbit
Contents
RNAse H2A Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RNAse H2A antibody was raised against a 17 amino acid synthetic peptide near the center of human RNAse H2A. The immunogen is located within amino acids 190 - 240 of RNAse H2A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
LANCL2 antibody is human, mouse and rat reactive.
Reactive Species
A07386 is reactive to RNASEH2A in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
33395 MW
Background of RNASEH2A
Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07386 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RNAse H2A antibody can be used for detection of RNAse H2A by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RNAse H2A in HeLa cell lysate with RNAse H2A antibody at 1 μg/mL.
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Immunocytochemistry of RNAse H2A in HeLa cells with RNAse H2A antibody at 2 μg/mL.
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Immunofluorescence of RNase H2A in Hela cells with RNAse H2A antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For RNASEH2A (Source: Uniprot.org, NCBI)
Gene Name
RNASEH2A
Full Name
Ribonuclease H2 subunit A
Weight
33395 MW
Superfamily
RNase HII family
Alternative Names
AGS4, JUNB, RNHL, RNHIA, RNASEHI, Ribonuclease H2 subunit A, Aicardi-Goutieres syndrome 4 protein, RNase H2 subunit A RNASEH2A AGS4, JUNB, RNASEHI, RNHIA, RNHL, THSD8 ribonuclease H2 subunit A ribonuclease H2 subunit A|RNase H(35)|RNase H2 subunit A|RNase HI large subunit|aicardi-Goutieres syndrome 4 protein|ribonuclease H2, large subunit|ribonuclease HI large subunit|ribonuclease HI subunit A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNASEH2A, check out the RNASEH2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNASEH2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNAse H2A Antibody (A07386)
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